M5257
Malic Dehydrogenase (oxalacetate-decarboxylating) from chicken liver
ammonium sulfate suspension, 10-30 units/mg protein (modified Warburg-Christian)
别名:
L-Malate:NADP+ oxidoreductase-(oxalacetate-decarboxylating), Malate Dehydrogenase (oxalacetate-decarboxylating), Malic Enzyme
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About This Item
一般描述
Malic dehydrogenase (MDH) exists as two isoforms within eukaryotic cells, one that is expressed in the mitochondria and functions in the TCA cycle and one in the cytoplasm that converts malate from the mitochondria back into oxaloacetate.
应用
Malic dehydrogenase has been used in a study to assess the dietary manganese requirement of juvenile yellow catfish (Pelteobagrus fulvidraco) and effects on whole body mineral composition and hepatic intermediary metabolism. It has also been used in a study to investigate the establishment and biological characterization of a fibroblast cell line from the Langshan chicken.
生化/生理作用
Expression of malic dehydrogenase in chicken liver is induced upon feeding and decreases in response to starvation.
单位定义
One unit will convert 1.0 μmole of L-malate and NADP to pyruvate, CO2, and NADPH per min at pH 7.4 at 25 °C.
外形
Suspension in 2.9 M (NH4)2SO4 solution containing 10 mM potassium phosphate, 0.5 mM 2-mercaptoethanol, 10 mM manganese chloride, and 3 mM Na4EDTA, pH 6.0
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
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