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M4655

Sigma-Aldrich

Eagle最低必需培养基

With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

别名:

MEM

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500 ML
¥430.88
1 L
¥647.25

¥430.88

预计发货时间2025年4月07日详情

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500 ML
¥430.88
1 L
¥647.25

About This Item

UNSPSC代码:
12352207
NACRES:
NA.75

¥430.88

预计发货时间2025年4月07日详情

获取大包装报价

无菌性

sterile-filtered

表单

liquid

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

sodium pyruvate: no
phenol red: yes
Earle’s salts (5% CO2): yes
HEPES: no
NaHCO3: yes
L-glutamine: yes

运输

ambient

储存温度

2-8°C

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此商品
M2414FG0325M0325
Eagle最低必需培养基 With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

M4655

Eagle最低必需培养基

Sigma-Aldrich

M2414

Eagle最低必需培养基

SAFC

FG0325

Minimum Essential Medium Eagle

Sigma-Aldrich

M0325

Eagle最低必需培养基

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

form

liquid

form

liquid

form

liquid

form

liquid

components

sodium pyruvate: no
phenol red: yes
Earle’s salts (5% CO2): yes
HEPES: no
NaHCO3: yes
L-glutamine: yes

components

phenol red: 0.011 g/L
NaHCO3: 0.85 g/L
glucose: 1.0 g/L
L-glutamine: no

components

stable glutamine: yes
L-glutamine: no
phenol red: yes
sodium pyruvate: no
Earle’s salts (5% CO2): yes
HEPES: no
Hanks’ salts (2% CO2): no
glucose: yes
NaHCO3: yes

components

glucose: 1.0 g/L (Dextro)
L-glutamine: 0.292 g/L
phenol red: 0.011 g/L
Earle’s salts (5% CO2): yes
NaHCO3: 2.2 g/L

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

一般描述

由Harry Eagle开发的最低必需培养基（MEM）是所有合成细胞培养基中使用最广泛的培养基之一。早期在尝试培养正常的哺乳动物成纤维细胞和HeLa细胞的某些亚型的过程中，显示它们具有Eagle's基础培养基（BME）无法满足的特定营养需求。随后在培养中使用这些细胞和其他细胞的研究表明，通过向BME中加入添加物以帮助更广泛的苛刻细胞的生长。

通过改良的MEM包括更高浓度的氨基酸，因此该培养基更接近于所培养的哺乳动物细胞的蛋白质组成。MEM已用于在单层中生长的各种细胞的培养。在含有Hanks'或Earle′s盐的配方中选择补充非必需氨基酸，可扩大该培养基的用途。

应用

通常用于在FBS、小牛或马血清存在下生长附着的细胞系，例如成纤维细胞。

其他说明

该产品中缺乏L-Ala; L-Asn; L-Glu; Gly; L-Pro; L-Ser。

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基
  • 技术规格说明书

    • 历史批次信息供参考:

    分析证书(COA)

    Lot/Batch Number
    RNBL9920
    RNBL9236
    RNBL9235
    RNBL7912
    RNBL7501

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