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安全信息

M4655

Sigma-Aldrich

Eagle最低必需培养基

With Earle′s salts, L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

别名:

MEM

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About This Item

UNSPSC代码:
12352207
NACRES:
NA.75

质量水平

400

无菌性

sterile-filtered

表单

liquid

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

sodium pyruvate: no
phenol red: yes
Earle’s salts (5% CO2): yes
HEPES: no
NaHCO3: yes
L-glutamine: yes

运输

ambient

储存温度

2-8°C

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相关类别

一般描述

由Harry Eagle开发的最低必需培养基（MEM）是所有合成细胞培养基中使用最广泛的培养基之一。早期在尝试培养正常的哺乳动物成纤维细胞和HeLa细胞的某些亚型的过程中，显示它们具有Eagle's基础培养基（BME）无法满足的特定营养需求。随后在培养中使用这些细胞和其他细胞的研究表明，通过向BME中加入添加物以帮助更广泛的苛刻细胞的生长。

通过改良的MEM包括更高浓度的氨基酸，因此该培养基更接近于所培养的哺乳动物细胞的蛋白质组成。MEM已用于在单层中生长的各种细胞的培养。在含有Hanks'或Earle′s盐的配方中选择补充非必需氨基酸，可扩大该培养基的用途。

应用

通常用于在FBS、小牛或马血清存在下生长附着的细胞系，例如成纤维细胞。

其他说明

该产品中缺乏L-Ala; L-Asn; L-Glu; Gly; L-Pro; L-Ser。

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

从最新的版本中选择一种：

分析证书(COA)

Lot/Batch Number
RNBL9920
RNBL9236
RNBL9235
RNBL7912
RNBL7501

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Jiaxin Hu et al.
Biochemistry, 53(28), 4510-4518 (2014-07-02)
Dentatorubral-pallidoluysian atrophy (DRPLA) is a progressive neurodegenerative disorder that currently has no curative treatments. DRPLA is caused by an expansion of a CAG trinucleotide repeat region within the protein-encoding sequence of the atrophin-1 (ATN-1) gene. Inhibition of mutant ATN-1 protein
Yongjun Chu et al.
Nucleic acids research, 48(12), 6740-6758 (2020-05-29)
How genetic defects trigger the molecular changes that cause late-onset disease is important for understanding disease progression and therapeutic development. Fuchs' endothelial corneal dystrophy (FECD) is an RNA-mediated disease caused by a trinucleotide CTG expansion in an intron within the
Mutsuyo Takayama-Ito et al.
PLoS neglected tropical diseases, 12(4), e0006398-e0006398 (2018-04-17)
Lymphocytic choriomeningitis virus (LCMV) causes a variety of diseases, including asymptomatic infections, meningitis, and congenital infections in the fetus of infected mother. The development of a safe and effective vaccine against LCMV is imperative. This study aims to develop a
Ivan Trus et al.
Frontiers in immunology, 10, 3077-3077 (2020-02-11)
Experimental increase of CpG dinucleotides in an RNA virus genome impairs infection providing a promising approach for vaccine development. While CpG recoding is an emerging and promising vaccine approach, little is known about infection phenotypes caused by recoded viruses in
Shelby Landreth et al.
Vaccines, 8(2) (2020-05-07)
Avian influenza H7N9 viruses continue to pose a great threat to public health, which is evident by their high case-fatality rates. Although H7N9 was first isolated in humans in China in 2013, to date, there is no commercial vaccine available
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