M3795
IgM, Kappa 来源于鼠骨髓瘤
clone TEPC 183, purified immunoglobulin, buffered aqueous solution
别名:
小鼠 IgM-κ
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关于此项目
Conjugate:
unconjugated
Clone:
TEPC 183, monoclonal
Application:
—
Citations:
22
biological source
mouse
conjugate
unconjugated
antibody form
purified immunoglobulin
clone
TEPC 183, monoclonal
assay
≥90% (HPLC)
form
buffered aqueous solution
concentration
2.0-2.2 mg/mL
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
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相关类别
General description
IgM抗体以五聚体的形式存在于血清中，其是通过对抗原的反应而产生的来源于鼠骨髓瘤的IgM, κ是由BAPC/c小鼠皮下注入的TEPC 183肿瘤细胞系产生的。
IgM是一种脊椎动物中高度保守的抗体，在免疫应答过程中早期表达。它是由腹膜B细胞分泌的。
通过280 nm (E1%/280 = 11.8)处吸光度测定含量，每瓶含有至少1 mg纯化骨髓瘤蛋白。
Application
来自小鼠骨髓瘤的IgM, Kappa已被用于：
- 夹心法酶联免疫吸附试验 (ELISA)
- 流式细胞术
- 斑点免疫结合试验
Biochem/physiol Actions
IgM在凋亡细胞的吞噬中起关键作用。血清IgM水平降低会导致自身免疫反应增强，并增加感染风险。IgM显示出多反应和自动反应功能。它可通过介导基于组织的分子的清除在组织动态平衡中起关键作用。IgM具有与N-连接的糖基化位点，其中糖是甘露糖、半乳糖、N-乙酰氨基葡萄糖和唾液酸。IgM连接的琼脂糖树脂经检测是有效的结合物结合。
TEPC 183肿瘤细胞系来源于原始诱导的浆细胞瘤，并在BALB/c小鼠皮下携带。TEPC 183细胞系的半抗原结合特异性尚未确定。它可特异性识别小鼠IgM。免疫球蛋白的识别性和纯度可通过免疫电泳进行确定。
Physical form
溶于含有0.05 M Tris(pH 8.0)、0.5 M氯化钠、0.02%叠氮化钠的溶液中
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
低风险生物材料
常规特殊物品
此项目有
T Sulimenko et al.
Journal of immunological methods, 289(1-2), 89-95 (2004-07-15)
Mouse monoclonal antibodies of IgG subclasses and IgM class in hybridoma culture supernatants were quantified using a dot-immunobinding assay. Immunoglobulins were bound to nitrocellulose (NC) membrane and, after blocking, the membrane was incubated with anti-mouse antibody conjugated to horseradish peroxidase
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Matthew P Collin et al.
Transplantation, 79(6), 722-725 (2005-03-24)
Graft-versus-host disease (GvHD), a life-threatening complication of bone marrow transplantation, is initiated by donor T cells reacting to recipient dendritic cells (DC). GvHD can be controlled by attenuating donor T cells, but few strategies exist to target DC, particularly resident
A fast and simple dot-immunobinding assay for quantification of mouse immunoglobulins in hybridoma culture supernatants
Sulimenko T and Draber P
Journal of Immunological Methods, 289(1-2), 89-95 (2004)
A-O Hovden et al.
Scandinavian journal of immunology, 62(4), 342-352 (2005-10-29)
We have previously found that whole influenza virus vaccine induced a more rapid and stronger humoral response, particularly after the first dose of vaccine, than split virus vaccine in mice. In this study, we have evaluated the protective efficacy of
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