M3682
Anti-MAP Kinase, Monophosphorylated Tyrosine antibody ,Mouse monoclonal
clone ERK-PY193, purified from hybridoma cell culture
别名:
Anti-pY-ERK
产品名称
Anti-MAP Kinase, Monophosphorylated Tyrosine antibody ,Mouse monoclonal, clone ERK-PY193, purified from hybridoma cell culture
biological source
mouse
conjugate
unconjugated
antibody form
purified from hybridoma cell culture
antibody product type
primary antibodies
clone
ERK-PY193, monoclonal
form
buffered aqueous solution
mol wt
antigen, ERK-1 44 kDa
antigen, ERK-2 42 kDa
species reactivity
rat, human
concentration
~2 mg/mL
technique(s)
capture ELISA: suitable
immunocytochemistry: suitable
microarray: suitable
western blot: 1-5 μg/mL using cell extract of rat fibroblasts cell line, Rat1, activated with sorbitol
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
phosphorylation (pTyr)
Gene Information
human ... MAPK1(5594), MAPK3(5595)
rat ... Mapk1(116590), Mapk3(50689)
Application
Monoclonal Anti-MAP Kinase, Monophosphorylated Tyrosine antibody has been used in:
- immunoblotting
- enzyme linked immunosorbent assay (ELISA)
- dot-blot
- immunocytochemistry
- western blotting
- immunofluorescence
Biochem/physiol Actions
MAPK kinase (MAPKK) is the immediate upstream activator of the MAPK, MAPKK kinase (MAP3K), and MAP3K kinase (MAP4K) for the enzymes further upstream, respectively. The kinases in the MAPK level are activated by phosphorylation of both tyrosine (Y) and threonine (T) residues organized in a TXY motif. The residue in between the two phosphorylated residues determines the specificity of activation of the MAPKs. Phosphorylation of both tyrosine and threonine is essential for the full activation of all MAPKs.
The antibody reacts with the monophosphorylated tyrosine form of MAP kinases (ERK1 and ERK2). It does not recognize the non-phosphorylated, diphosphorylated, and the monophosphorylated threonine forms of ERK/MAP kinases, or the diphosphorylated forms of JNK and p38 MAPK. The epitope recognized by the antibody contains the phosphorylated tyrosine residue within the regulatory site of MAP kinase (e.g.,Tyr185 in ERK-2). Cross-reactivity has been observed with the monophosphorylated tyrosine peptide of JNK.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
General description
Monoclonal Anti-MAP Kinase, Mono-phosphorylated Tyrosine (pY-ERK) (mouse IgG1 isotype) is derived from the ERK-PY193 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide sequence corresponding to the phosphorylated form of ERK-activation loop, conjugated to KLH. The mitogen-activated protein kinase (MAPK) superfamily of enzymes is involved in many signaling pathways. This family includes the ERK1/2 (extracellular signal-regulated protein kinase, also termed p42/p44 MAPK), JNK (c-Jun N-terminal protein kinase, also termed stress-activated protein kinase, SAPK1), and p38 MAPK (also termed SAPK2) subfamilies.
Immunogen
synthetic peptide HTGFLTEpYVAT, corresponding to the phosphorylated form of ERK-activation loop.
Physical form
Solution in phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
Preparation Note
Prepared from a culture supernatant of bioreactor grown hybridoma.
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存储类别
10 - Combustible liquids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
常规特殊物品
此项目有
Mammalian MAP kinase signalling cascades
Chang L and Karin M
Nature, 410(6824), 37-37 (2001)
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Mathematical models are extensively employed to understand physicochemical processes in biological systems. In the absence of detailed mechanistic knowledge, models are often based on network inference methods, which in turn rely upon perturbations to nodes by biochemical means. We have
Paradoxical results in perturbation-based signaling network reconstruction
Prabakaran S, et al.
Biophysical Journal, 106(12), 2720-2728 (2014)
H Cha et al.
The Journal of cell biology, 153(7), 1355-1367 (2001-06-27)
Phosphorylation of the extracellular signal-regulated kinases (ERKs) on tyrosine and threonine residues within the TEY tripeptide motif induces ERK activation and targeting of substrates. Although it is recognized that phosphorylation of both residues is required for ERK activation, it is
Monophosphothreonyl extracellular signal-regulated kinases 1 and 2 (ERK1/2) are formed endogenously in intact cardiac myocytes and are enzymically active.
Sugden PH, et al.
Cellular Signalling, 23(2), 468-477 (2011)
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