生物来源
rabbit
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
分子量
antigen 160 kDa
种属反应性
human
包装
antibody small pack of 25 μL
浓度
~1 μg/L
技术
immunoprecipitation (IP): 2.5-5 μg using HEK 293T cell lysates
indirect immunofluorescence: 10-20 μg/mL using paraformaldehyde-fixed HeLa cells
microarray: suitable
western blot: 0.5-1 μg/mL using HEK 293T cell lysates
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... MSH6(2956)
一般描述
MutS Homolog 6 (MSH6) is localized on human chromosome 2p16.3. MSH6 is a 160 kDa protein with nuclear localization sequence and a N-terminal disordered domain. It is a human homolog of E. coli MutS.
免疫原
synthetic peptide corresponding to amino acids 1311-1327 of human MSH6, conjugated to KLH via an N-terminal added cysteine residue. The immunizing peptide differs from the rat and mouse sequences in one amino acid.
应用
Anti-MSH6 (C-terminal) antibody produced in rabbit has been used in:
- immunoblotting
- immunoprecipitation
- immunofluorescence
生化/生理作用
MSH6 (also known as GTBP) was isolated from HeLa cells by virtue of its ability to restore mismatch repair to nuclear extracts of MSH2-deficient colorectal tumor cell lines. MutS Homolog 6 (MSH6) is part ofDNA repair pathways and play a key role onmutator S protein (hMutSα) shuttling. MSH6 has ATPase activity that is critical in mismatch repair. Germline mutations in MSH6 is associated with hereditary nonpolyposis colon cancer and in endometrial carcinoma.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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法规信息
常规特殊物品
Prevalence of defective DNA mismatch repair and MSH6 mutation in an unselected series of endometrial cancers
Goodfellow PJ, et al.
Proceedings of the National Academy of Sciences of the USA, 100(10), 5908-5913 (2003)
Structural, molecular and cellular functions of MSH2 and MSH6 during DNA mismatch repair, damage signaling and other noncanonical activities
Edelbrock MA, et al.
Mutation Research. Fundamental and Molecular Mechanisms of Mutagenesis, 743, 53-66 (2013)
Inhibition of Msh6 ATPase activity by mispaired DNA induces a Msh2 (ATP)-Msh6 (ATP) state capable of hydrolysis-independent movement along DNA
Mazur DJ
Molecular Cell, 22(1), 39-49 (2006)
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