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安全信息

M2634

Sigma-Aldrich

苹果酸脱氢酶 来源于猪心脏

buffered aqueous glycerol solution, 600-1000 units/mg protein (biuret)

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别名:
L-苹果酸: NAD+ 氧化还原酶, MDH, Malate Dehydrogenase
EC 号:
1.1.1.37 (BRENDA, IUBMB)
MDL编号:
MFCD00131567
UNSPSC代码:
12352204
NACRES:
NA.54

形式

buffered aqueous glycerol solution

质量水平

200

比活

600-1000 units/mg protein (biuret)

痕量阳离子

NH4+: ≤10 μg/mg protein

异质活性

Glutamic-Oxalacetic Transaminase ≤0.01%
Glutamic-Pyruvic Transaminase ≤0.01%

运输

wet ice

储存温度

2-8°C

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一般描述

线粒体同工酶
苹果酸脱氢酶是线粒体同工酶，也是三羧酸循环中的重要催化剂。

应用

采用苹果酸脱氢酶对银杏提取物对寄主钉螺的软体动物的杀灭作用进行了比较研究。 它还被用于研究早期饲养限制对肉鸡代谢程序和补偿性生长的影响。

生化/生理作用

真核细胞含有两种不同的苹果酸脱氢酶同工酶：线粒体同工酶（m-MDH）和可溶性或细胞质同工酶（s-MDH）。该产品由线粒体同工酶形式组成。
该酶可催化以下反应：草酰乙酸+β-NADH→ L-苹果酸+β-NAD
通过在25℃下用5′-磷酸吡哆醛处理可以可逆地对苹果酸脱氢酶进行灭活。

单位定义

在pH7.5,25℃条件下，一个单位每分钟可将1.0 μmole的草酰乙酸和β-NADH转化为 L苹果酸和β-NAD。

外形

溶于含50％甘油的0.05M磷酸钾缓冲液（pH7.5）中

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

动植物源性产品

分析证书(COA)

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实验方案

Enzymatic Assay of Malic Dehydrogenase

Spectrophotometric assay evaluates malic dehydrogenase activity using bovine heart enzyme with critical histidine residue at active site.

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