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表单
buffered aqueous glycerol solution
质量水平
比活
600-1000 units/mg protein (biuret)
痕量阳离子
NH4+: ≤10 μg/mg protein
异质活性
Glutamic-Oxalacetic Transaminase ≤0.01%
Glutamic-Pyruvic Transaminase ≤0.01%
运输
wet ice
储存温度
2-8°C
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一般描述
线粒体同工酶
苹果酸脱氢酶是线粒体同工酶，也是三羧酸循环中的重要催化剂。
应用
采用苹果酸脱氢酶对银杏提取物对寄主钉螺的软体动物的杀灭作用进行了比较研究。 它还被用于研究早期饲养限制对肉鸡代谢程序和补偿性生长的影响。
生化/生理作用
真核细胞含有两种不同的苹果酸脱氢酶同工酶：线粒体同工酶（m-MDH）和可溶性或细胞质同工酶（s-MDH）。该产品由线粒体同工酶形式组成。
该酶可催化以下反应：草酰乙酸+β-NADH→ L-苹果酸+β-NAD
该酶可催化以下反应：草酰乙酸+β-NADH→ L-苹果酸+β-NAD
通过在25℃下用5′-磷酸吡哆醛处理可以可逆地对苹果酸脱氢酶进行灭活。
单位定义
在pH7.5,25℃条件下，一个单位每分钟可将1.0 μmole的草酰乙酸和β-NADH转化为 L苹果酸和β-NAD。
外形
溶于含50％甘油的0.05M磷酸钾缓冲液（pH7.5）中
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
动植物源性产品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Comparative molluscicidal action of extract of Ginko biloba sarcotesta, arecoline and niclosamide on snail hosts of Schistosoma japonicum
Chen, S., et al.
Pesticide Biochemistry and Physiology, 89, 237-241 (2007)
X A Zhan et al.
Poultry science, 86(4), 654-660 (2007-03-21)
The effect of early feed restriction on metabolic programming and compensatory growth was studied in broiler chickens. A total of 480 female 1-d-old broiler birds (Aconred) were randomly allocated to ad libitum and feed-restricted groups, each of which was replicated
S S Chen et al.
The Biochemical journal, 151(2), 297-303 (1975-11-01)
1. Pig heart mitochondrial malate dehydrogenase incubated with pyridoxal 5'-phosphate at pH 8.0 and 25 degrees C gradually loses activity. Such inactivation can be largely reversed by dialysis or by addition of L-lysine or L-cysteine, and can be made permanent
Nicholas R Moody et al.
Bio-protocol, 11(24), e4264-e4264 (2022-01-29)
Phosphoenolpyruvate carboxylase (PEPC) catalyzes a critical step in carbon metabolism in plants and bacteria, the irreversible reaction between bicarbonate and phosphoenolpyruvate to produce the C4 compound oxaloacetate. This enzyme is particularly important in the context of C4 photosynthesis, where it
Tatyana Almeida Tavella et al.
ACS infectious diseases, 7(4), 759-776 (2021-03-11)
Antimalarial drugs with novel modes of action and wide therapeutic potential are needed to pave the way for malaria eradication. Violacein is a natural compound known for its biological activity against cancer cells and several pathogens, including the malaria parasite
实验方案
Spectrophotometric assay evaluates malic dehydrogenase activity using bovine heart enzyme with critical histidine residue at active site.
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