M2154
培养基 199
With Earle′s salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture
别名:
M199 Medium, TCM 199
产品名称
培养基 199, With Earle′s salts and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
sterility
sterile-filtered
form
liquid
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin, tested
pH
7.0-7.6 (25 °C, 209 g/L)
components
sodium pyruvate: no
L-glutamine: no
NaHCO3: yes
HEPES: no
phenol red: yes
Earle’s salts (5% CO2): yes
shipped in
ambient
storage temp.
2-8°C
Quality Level
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Application
培养基199已用于：
- 培养一岁Speckle Park母牛来源的瘤胃上皮细胞
- 作为成熟培养基组分，培养牛卵巢来源的卵丘卵母细胞复合体(COC)
- 作为杜氏改良Eagle培养基(DMEM)组分，维持新生大鼠心肌细胞(NCM)生长
General description
培养基199是维生素、氨基酸和其他因素的组合，最初开发为用于原代外植体培养的完全确定的培养基配方。这种培养基添加适当的补充剂时具有广泛的适用性，特别是对于非转化细胞。培养基 199 广泛用于疫苗生产、原代胰腺外植体和晶状体组织的体外培养。
Preparation Note
补充 0.1 g/L L-谷氨酰胺。
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
动植物来源培养基
此项目有
E Held-Hoelker et al.
Theriogenology, 96, 145-152 (2017-05-24)
Lipid accumulation is associated with reduced embryonic quality, causing limited survival after cryopreservation. Therefore, in the present study we aimed to reveal the effects of supplementation of a lipid reducing agent, l-carnitine and the removal of fatty acids during in vitro
C Kent-Dennis et al.
Journal of dairy science, 104(1), 1002-1017 (2020-11-03)
Inflammation of ruminal epithelium may occur during ruminal acidosis as a result of translocation and interaction of ruminal epithelial cells (REC) with molecules such as lipopolysaccharide (LPS). Such inflammation has been reported to alter cellular processes such as nutrient absorption
Melisa Gomez-Velazquez et al.
PLoS genetics, 13(8), e1006985-e1006985 (2017-08-29)
Cardiac progenitors are specified early in development and progressively differentiate and mature into fully functional cardiomyocytes. This process is controlled by an extensively studied transcriptional program. However, the regulatory events coordinating the progression of such program from development to maturation
Michelle M Halstead et al.
Nature communications, 11(1), 4654-4654 (2020-09-19)
The shift from maternal to embryonic control is a critical developmental milestone in preimplantation development. Widespread transcriptomic and epigenetic remodeling facilitate this transition from terminally differentiated gametes to totipotent blastomeres, but the identity of transcription factors (TF) and genomic elements
Alfred Nguyen et al.
Lab on a chip, 21(10), 1929-1947 (2021-05-20)
Active microrheology is one of the main methods to determine the mechanical properties of cells and tissue, and the modelling of these viscoelastic properties is under heavy debate with many competing approaches. Most experimental methods of active microrheology such as
商品
Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.
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