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Merck
CN

M1570

Anti-MYH-1 Antibody

mouse monoclonal, MY-32

别名:

单克隆抗肌球蛋白（骨骼肌快肌） 小鼠抗

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关于此项目

UNSPSC Code:
12352203
NACRES:
NA.41
MDL number:
MFCD00145920
Conjugate:
unconjugated
Clone:
MY-32, monoclonal
Application:
immunohistochemistry (formalin-fixed, paraffin-embedded sections)
microarray
western blot
Species reactivity:
rat, chicken, rabbit, mouse, human, bovine, guinea pig, feline
Citations:
30
Technique(s):
immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10-20 μg/mL using porcine tongue
microarray: suitable
western blot: 0.5-1.0 μg/mL using total extract of rabbit skeletal muscle
Uniprot accession no.:
P12882

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产品名称

抗肌球蛋白(骨骼肌,快速)抗体，小鼠单克隆抗体, clone MY-32, purified from hybridoma cell culture

biological source

mouse

conjugate

unconjugated

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

MY-32, monoclonal

form

buffered aqueous solution

species reactivity

rat, chicken, rabbit, mouse, human, bovine, guinea pig, feline

packaging

antibody small pack of 25 μL

enhanced validation

independent
Learn more about Antibody Enhanced Validation

concentration

~1.0 mg/mL

technique(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 10-20 μg/mL using porcine tongue
microarray: suitable
western blot: 0.5-1.0 μg/mL using total extract of rabbit skeletal muscle

isotype

IgG1

UniProt accession no.

P12882

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Quality Level

200

Gene Information

human ... MYH1(4619), MYH2(4620)
mouse ... Myh1(17879), Myh2(17882)
rat ... Myh1(287408), Myh2(691644)

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相关类别

Application

使用单克隆小鼠抗肌球蛋白（骨骼/快速）作为一抗，以1：90000的稀释度，通过蛋白质印迹对来自具有过去损伤的运动员的血清样品中的肌球蛋白（快）水平进行测定。
成功使用该抗体的应用以及相关的同行评审论文如下所示。
免疫组织化学（1篇论文）

Disclaimer

除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

General description

定位于肌球蛋白重链上的表位。可对骨骼肌中发现的快速（II型）和新生儿肌动蛋白分子进行染色，但不会对培养细胞中的心肌、平滑肌或非肌肉肌球蛋白染色。能与人类横纹肌肉瘤发生反应。

Immunogen

兔肌肉肌球蛋白。

Physical form

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

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存储类别

10 - Combustible liquids

wgk

WGK 1

法规信息

常规特殊物品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000521934
0000521934
0000492452
0000492452
0000458272

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Charlotte Capitanchik et al.
Nucleus (Austin, Tex.), 9(1), 410-430 (2018-06-19)
Laminopathies yield tissue-specific pathologies, yet arise from mutation of ubiquitously-expressed genes. A little investigated hypothesis to explain this is that the mutated proteins or their partners have tissue-specific splice variants. To test this, we analyzed RNA-Seq datasets, finding novel isoforms
Yanlin Wang et al.
Neurological sciences : official journal of the Italian Neurological Society and of the Italian Society of Clinical Neurophysiology, 40(6), 1255-1265 (2019-03-21)
Myotonic dystrophy type 1 (DM1) is caused by CTG nucleotide repeat expansions in the 3'-untranslated region (3'-UTR) of the dystrophia myotonica protein kinase (DMPK) gene. The expanded CTG repeats encode toxic CUG RNAs that cause disease, largely through RNA gain-of-function.
Alan P Tenney et al.
Cell reports, 29(2), 437-452 (2019-10-10)
The somatotopic motor-neuron projections onto their cognate target muscles are essential for coordinated movement, but how that occurs for facial motor circuits, which have critical roles in respiratory and interactive behaviors, is poorly understood. We report extensive molecular heterogeneity in
Shirin Pourteymour et al.
Physiological reports, 3(8), doi:10-doi:10 (2015-08-13)
Perilipins (PLINs) coat the surface of lipid droplets and are important for the regulation of lipid turnover. Knowledge about the physiological role of the individual PLINs in skeletal muscle is limited although lipid metabolism is very important for muscle contraction.
Emranul Huq et al.
American journal of physical anthropology, 166(1), 95-106 (2018-01-11)
We hypothesized that the vertical leaper Galago senegalensis will have epaxial extensor muscles with a fast fiber phenotype to facilitate rapid spinal extension during leaping in comparison to the slow-moving quadruped Nycticebus coucang. To test this, we determined the percentage
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