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Merck
CN

M0650

Sigma-Aldrich

培养基 199

With Earle′s salts, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture, 10X

别名:

Cell growth medium, M199 Medium, TCM 199

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About This Item

UNSPSC代码:
12352207
NACRES:
NA.75

产品名称

培养基 199, 10 ×, With Earle′s salts, without L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

质量水平

无菌性

sterile-filtered

表单

liquid

浓度

10 ×

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

NaHCO3: no
phenol red: yes
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO2): yes
L-glutamine: no

运输

ambient

储存温度

2-8°C

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一般描述

培养基199是维生素、氨基酸和其他因素的组合,最初开发为用于原代外植体培养的完全确定的培养基配方。这种培养基添加适当的补充剂时具有广泛的适用性,特别是对于非转化细胞。培养基 199 广泛用于疫苗生产、原代胰腺外植体和晶状体组织的体外培养。

应用

培养基199已用于:

  • 培养人间皮细胞
  • 作为保存培养基的组分,用于收集多产布尔山羊胚胎
  • 制备球形包埋的胶原基质

重悬

补充0.1 g/L L-谷氨酰胺,2.2 g/L碳酸氢钠(1×)。

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 2

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

从最新的版本中选择一种:

分析证书(COA)

Lot/Batch Number

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商品

Medium 199 supports non-transformed cell cultivation in virology and vaccine production, offering broad species applicability.

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