product name
StableCell™ MEM, With Earle′s salts, stable glutamine, and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
质量水平
无菌性
sterile-filtered
形式
liquid
技术
cell culture | mammalian: suitable
杂质
endotoxin, tested
组分
sodium pyruvate: no
HEPES: no
Hanks’ salts (2% CO2): no
NaHCO3: yes
Earle’s salts (5% CO2): no
stable glutamine: yes
phenol red: yes
L-glutamine: no
glucose: yes
运输
ambient
储存温度
2-8°C
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一般描述
由 Harry Eagle 开发的最低必需培养基（MEM）是所有合成细胞培养基中使用最广泛的培养基之一。
由 Harry Eagle 开发的最低必需培养基（MEM）是所有合成细胞培养基中使用最广泛的培养基之一。正常哺乳动物成纤维细胞和某些亚型的 HeLa 细胞的早期尝试培养表明,Eagle 基础培养基（BME）无法满足的他们的特殊营养需求。随后培养这些和其他细胞的研究表明，在 BME 中补充添加剂有利于更多种类的苛求细胞的生长。
MEM 包含了这些改进，包括更高浓度的氨基酸，因此培养基更接近于培养的哺乳动物细胞的蛋白质组成。MEM 已用于多种单层细胞的培养。在含有 Hanks 或 Earle 盐的配方中可选地补充非必需氨基酸，扩大了该培养基的用途。
MEM 包含了这些改进，包括更高浓度的氨基酸，因此培养基更接近于培养的哺乳动物细胞的蛋白质组成。MEM 已用于多种单层细胞的培养。在含有 Hanks 或 Earle 盐的配方中可选地补充非必需氨基酸，扩大了该培养基的用途。
应用
低限量 Eagle 培养基已用于小鼠成纤维细胞培养，用于体外细胞相容性测试。
其他说明
该产品不含 L-丙氨酸；L-天冬酰胺；L-谷氨酸；甘氨酸；L-脯氨酸；L-丝氨酸 和 L-谷氨酰胺。该产品补充了 L-Ala-L-Gln 二肽。这为细胞培养提供了更稳定的谷氨酰胺形式。游离氨基酸 L-谷氨酰胺在细胞培养中是不稳定的。
法律信息
StableCell is a trademark of Sigma-Aldrich Co. LLC
推荐
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产品编号
说明
价格
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves
法规信息
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