跳转至内容
Merck
CN
所有图片(1)

文件

安全信息

M042080

Sigma-Aldrich

Biotin Phosphoramidite

configured for PerkinElmer, configured for Polygen

登录查看公司和协议定价
所有图片(1)
别名:
生物素酰胺
经验公式（希尔记法）:
C46H64N5O8PS
CAS号:
1217500-22-5
分子量:
878.07
MDL编号:
MFCD00676535
UNSPSC代码:
41116105
PubChem化学物质编号:
329817781
NACRES:
NA.21

产品线

Proligo Reagents

质量水平

200

检测方案

≥96% (31P-NMR)

适用性

conforms to structure for H-NMR

相容性

configured for PerkinElmer
configured for Polygen

储存温度

−20°C

SMILES字符串

COc1ccc(cc1)C(N2C3CS[C@@H](CCCCC(=O)NCCOCCOCCOP(OCCC#N)N(C(C)C)C(C)C)C3NC2=O)(c4ccccc4)c5ccc(OC)cc5

InChI

1S/C46H64N5O8PS/c1-34(2)51(35(3)4)60(58-27-12-25-47)59-32-31-57-30-29-56-28-26-48-43(52)16-11-10-15-42-44-41(33-61-42)50(45(53)49-44)46(36-13-8-7-9-14-36,37-17-21-39(54-5)22-18-37)38-19-23-40(55-6)24-20-38/h7-9,13-14,17-24,34-35,41-42,44H,10-12,15-16,26-33H2,1-6H3,(H,48,52)(H,49,53)/t41-,42-,44-,60?/m0/s1

InChI key

WSDQYLGSIUJJRU-ZMKGMYJVSA-N

正在寻找类似产品? 访问 产品对比指南

相关类别

一般描述

Biotin phosphoramidite is a reagent used to directly incorporate a biotin label at the 5′ hydroxy group of the terminal nucleoside base through a p-aminophenethyl spacer group during oligonucleotide synthesis.

The photocleavable biotin phosphoramidite reagent is compatible with automated DNA/RNA synthesizers using phosphoramidite chemistry. The length of the p-aminophenethyl spacer group allows streptavidin affinity purification of the oligonucleotide by reverse-phase HPLC. Biotinlabeling is compatible with PCR and most hybridization techniques.Key Features of Biotin Phosphoramidite
  • Behaves like any standard phosphoramidite on the DNA synthesizer
  • Readily soluble in acetonitrile
  • Biotin compound has a dimethoxytrityl (DMT) group on the ring structure,which allows coupling-yield determination and trityl-selective purification• A long, water-compatible linker arm ensures maximum sensitivity
  • Provides a coupling efficiency of at least 95%The product is configured for Expedite™ and PolyGen® Synthesizers.

应用

Biotin Phosphoramidite is suitable for the synthesis of 5′-biotinylated oligonucleotides whose applications are seen in ligase-mediated gene detection, direct dideoxy sequencing following PCR, and non-radioactive sequencing of DNA.

法律信息

Expedite is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
PolyGen is a registered trademark of PolyGen GmbH.

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

新产品

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门