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安全信息

M0275

Sigma-Aldrich

Eagle最低必需培养基

With Earle′s salts, without ʟ-glutamine and sodium bicarbonate, sterile liquid, suitable for cell culture, 10x

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别名:
MEM
UNSPSC代码:
12352207
NACRES:
NA.71

质量水平

400

无菌性

sterile-filtered

形式

liquid

浓度

10 ×

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested

组分

phenol red: yes
HEPES: no
Earle’s salts (5% CO2): yes
L-glutamine: no
sodium pyruvate: no
NaHCO3: no

运输

ambient

储存温度

2-8°C

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应用

最低必需培养基 Eagle 已用于配制甲基丙烯酸丙烯酰胺（水凝胶）。它也已用于培养牛膝关节的软骨，随后该牛膝关节软骨可用于 MRI（磁共振成像）研究和组织学分析。
通常用于在FBS、小牛或马血清存在下培养贴壁细胞，如成纤维细胞。待培养基稀释到1x和调节pH后，再添加L-谷氨酰胺和碳酸氢钠。

重悬

向 1× 培养基中添加 0.292g/L L-谷氨酰胺、2.2g/L 碳酸氢钠。

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WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

动植物来源培养基

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MEM (Minimum Essential Medium Eagle)

A large selection of MEM formulations. Minimum Essential Medium (MEM), developed by Harry Eagle, is one of the most widely used of all synthetic cell culture media.

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