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Merck
CN

L9764

Sigma-Aldrich

脂多糖(粗糙菌株) 来源于肠沙门氏菌 明尼苏达血清型 Re 595(Re 突变体)

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别名:
LPS
MDL编号:
UNSPSC代码:
12352201
NACRES:
NA.25

生物来源

Salmonella enterica (Serotype minnesota Re 595 mutant)

质量水平

形式

lyophilized powder

杂质

<3% Protein (Lowry)

颜色

white to yellow cast

溶解性

water: 0.90-1.10 mg/mL, hazy to turbid, colorless to faintly yellow

运输

ambient

储存温度

2-8°C

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一般描述

本品由沙门氏菌菌种明尼苏达粒径Re 595(Re突变体)采用酚-氯仿-石油醚提取程序制备而成。来源菌株为 ATCC 49284。菌株 Re 595 的 LPS 中的一种独特结构显示可诱导人血小板分泌和聚集。

应用


  • Charge and aggregation pattern govern the interaction of plasticins with LPS monolayers mimicking the external leaflet of the outer membrane of Gram-negative bacteria: This study examines how charge and aggregation patterns affect the interaction of plasticins with lipopolysaccharides (LPS) from rough strains like Salmonella enterica serotype Minnesota Re 595. The findings contribute to understanding the molecular interactions involved in microbial resistance mechanisms (Michel et al., 2015).

生化/生理作用

脂多糖(LPS)位于膜的外层,并且在非包封的菌株中,其暴露于细胞表面。它们有助于外膜的完整性,并可保护细胞免受胆汁盐和亲脂性抗生素的作用。

制备说明

通过苯酚-氯仿-石油醚萃取进行制备。

本品可溶于水(5 mg/ml)或细胞培养基(1 mg/ml),产生混浊的淡黄色溶液。溶于生理盐水并涡旋和加热至70-80 oC后,可形成浓度更高但仍然混浊的溶液(20 mg/ml)。脂多糖分子可在各种溶剂中形成胶束。溶于水和磷酸盐缓冲盐水时,可观察到浑浊的溶液。使用有机溶剂不能形成更清晰的溶液。使用甲醇可产生具有漂浮物的混浊悬浮液,而溶于水会产生均匀的混浊溶液。

其他说明

为了全面了解我们针对客户研究提供的各种脂多糖产品,建议您访问我们的碳水化合物分类页面。

象形图

Exclamation mark

警示用语:

Warning

危险声明

预防措施声明

危险分类

Acute Tox. 4 Oral

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Gloves


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Journal of immunology (Baltimore, Md. : 1950), 188(7), 3382-3394 (2012-03-07)
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