生物来源
Escherichia coli (EH100 Ra mutant)
质量水平
形式
lyophilized powder
杂质
<3% Protein (Lowry)
颜色
white to yellow
溶解性
water: 4.90-5.10 mg/mL, cloudy to turbid, colorless to faintly yellow
运输
ambient
储存温度
2-8°C
一般描述
本品是使用苯酚:氯仿:石油醚从大肠杆菌EH 100（Ra突变株）中提取的。来源菌株由私人提供。
生化/生理作用
脂多糖（LPS）位于无荚膜菌株的膜外层，暴露于细胞表面。它们有助于维持外膜的完整性，并防止胆汁盐和亲脂性抗生素破坏细胞。
制备说明
通过苯酚-氯仿-石油醚萃取进行制备。
本品可溶于水（5 mg/ml）或细胞培养基（1 mg/ml），产生混浊的淡黄色溶液。溶于生理盐水并涡旋和加热至70-80 oC后，可形成浓度更高但仍然混浊的溶液（20 mg/ml）。脂多糖分子可在各种溶剂中形成胶束。溶于水和磷酸盐缓冲盐水时，可观察到浑浊的溶液。使用有机溶剂不能形成更清晰的溶液。使用甲醇可产生具有漂浮物的混浊悬浮液，而溶于水会产生均匀的混浊溶液。
本品可溶于水（5 mg/ml）或细胞培养基（1 mg/ml），产生混浊的淡黄色溶液。溶于生理盐水并涡旋和加热至70-80 oC后，可形成浓度更高但仍然混浊的溶液（20 mg/ml）。脂多糖分子可在各种溶剂中形成胶束。溶于水和磷酸盐缓冲盐水时，可观察到浑浊的溶液。使用有机溶剂不能形成更清晰的溶液。使用甲醇可产生具有漂浮物的混浊悬浮液，而溶于水会产生均匀的混浊溶液。
警示用语：
Danger
危险声明
危险分类
Acute Tox. 2 Oral
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
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