推荐产品
生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
形式
buffered aqueous solution
分子量
antigen 16-18 kDa
种属反应性
mouse, human, rat
包装
antibody small pack of 25 μL
浓度
~1 mg/mL
技术
immunohistochemistry: suitable
immunoprecipitation (IP): 5-10 μg using extracts of human U87 cells
western blot: 1-2 μg/mL using whole extracts of rat and mouse brain
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... MAP1LC3A(84557) , MAP1LC3B(81631)
mouse ... Map1lc3a(66734) , Map1lc3b(67443)
rat ... Map1lc3a(362245) , Map1lc3b(64862)
相关类别
一般描述
LC3基因,也称为MAP1LC3A(微管相关蛋白1轻链3 α),是酵母自噬所必需的Apg8p同源物。处理后形成的胞质可溶形式定位于自噬体膜上。该基因位于人染色体20q11.22上。LC3在细胞中以细胞质LC3-I(18kDa)和LC3-II(16kDa)两种形式存在。
免疫原
该合成肽对应于人LC3A亚型a的氨基酸,通过C-末端半胱氨酸残基与KLH偶联。在大鼠和小鼠中的相应序列是相同的。
应用
兔抗LC3 抗体可用于:
- 蛋白质印迹
- 免疫荧光
- 免疫组化
- 过滤延迟测定
- 免疫细胞化学
生化/生理作用
MAP1LC3A基因(微管相关蛋白1轻链3 α)编码自噬体标记蛋白。已有研究发现它在Nasu-Hakola病大脑中幸存的少突胶质细胞中表达,并且可能参与诱导少突神经胶质细胞病。
外形
0.01M 磷酸缓冲盐溶液,pH 7.4,含 15mM 叠氮化钠。
储存及稳定性
若需连续使用,可在2-8°C下保存最长一个月。若需延长储存时间,可将溶液分装并冷冻。不建议反复冻融。如果长期储存时出现轻微浑浊,请在使用前通过离心澄清溶液。若工作稀释样品在12小时内未使用完,则应丢弃。
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
WGK
WGK 3
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
常规特殊物品
TRIAD1 Is a Novel Transcriptional Target of p53 and Regulates Nutlin-3a-Induced Cell Death.
Journal of Cellular Biochemistry (2016)
LC3, an autophagosome marker, is expressed on oligodendrocytes in Nasu-Hakola disease brains.
Orphanet Journal of Rare Diseases, 9 (2014)
PloS one, 10(12), e0143922-e0143922 (2015-12-05)
We investigated the mechanisms underlying damage to rat small intestine in heat- and shake-induced stress. Eighteen Sprague-Dawley rats were randomly divided into a control group and a 3-day stressed group treated 2 h daily for 3 days on a rotary
The international journal of biochemistry & cell biology, 113, 48-57 (2019-05-28)
Treatment outcomes for hepatocellular carcinoma (HCC) remain unsatisfactory, and effective new therapeutic methods are urgently needed. Gossypol has been shown to have an anti-HCC effect, but the underlying mechanism requires further study. In this study, we found gossypol inhibited HCC
Molecular pharmaceutics, 10(1), 270-277 (2012-12-06)
Chronic myeloid leukemia (CML) is a myeloproliferative disorder caused by expression of the fusion gene BCR-ABL following a chromosomal translocation in the hematopoietic stem cell. Therapeutic management of CML uses tyrosine kinase inhibitors (TKIs), which block ABL-signaling and effectively kill
商品
High titer lentiviral particles for LC3 variants used for live cell analysis of cellular autophagy.
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