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一般描述
凝集素是植物来源的蛋白质,其具有结合某些神经元表面糖蛋白和糖脂的能力。他们的这种能力有助于它们被用作神经解剖学示踪剂,因为它们以逆行或顺行方式被采集和运输。
应用
来自美洲商陆(商陆)的凝集素用于增殖测定。(使用常规测定,通过在新合成的DNA中掺入 3H 胸苷来测量细胞增殖反应)。
生化/生理作用
凝集素是碳水化合物结合蛋白,对糖部分具有高度特异性。商陆凝集素(PL)是对含有N-乙酰氨基葡糖的糖特异的凝集素,其通过与细胞表面结合来刺激外周淋巴细胞进行有丝分裂。从商陆(美洲商陆)的根中分离出四种类型的凝集素。它们含有同源结构域,但具有不同的分子大小和生物学特性。
PWM 从商陆根中提取。该蛋白质具有红血球凝集、白血球凝集和促有丝分裂特性。PWM 不具有血型特异性,但具有与 N-乙酰基-β-D-葡糖胺低聚物的亲和性。
质量
部分纯化的 TCA 沉淀。
基本不含盐。
基本不含盐。
分析说明
通过将 BrdU 掺入 HeLa 细胞培养基中测定有丝分裂促进活性。
警示用语:
Danger
危险声明
危险分类
Resp. Sens. 1 - Skin Sens. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
动植物源性产品
Animals : an open access journal from MDPI, 11(10) (2021-10-24)
Immunological studies in the horse are frequently hampered by lack of environmental control, complicated study design, and ethical concerns when performing high risk studies. The purpose of the current study was to investigate the utility of a xenograft model for
Acta crystallographica. Section D, Biological crystallography, 60(Pt 4), 665-673 (2004-03-25)
Pokeweed lectin (PL), a lectin specific for N-acetylglucosamine-containing saccharides, stimulates peripheral lymphocytes to undergo mitosis by binding to their cell surfaces. Four types of lectins have been isolated from the roots of pokeweed (Phytolacca americana) and shown to contain homologous
Scientific reports, 10(1), 7471-7471 (2020-05-06)
In the routine commercial karyotype analysis on 5,481 boars, we identified 32 carriers of mosaic reciprocal translocations, half of which were carrying a specific recurrent translocation, mos t(7;9). An additional 7 mosaic translocations were identified through lymphocyte karyotype analysis from
Brain research, 290(2), 219-238 (1984-01-09)
A new neuroanatomical method for tracing connections in the central nervous system based on the anterograde axonal transport of the kidney bean lectin, Phaseolus vulgaris-leucoagglutinin (PHA-L) is described. The method, for which a detailed protocol is presented, offers several advantages
Kidney international, 78(4), 396-407 (2010-05-21)
Immunoglobulin A nephropathy (IgAN) is the most common form of primary glomerulonephritis worldwide. The basic defect lies within the IgA immune system and in peripheral blood leukocytes, rather than local kidney abnormalities. To define the intracellular mechanisms leading to the
商品
Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.
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