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Merck
CN

L8629

Sigma-Aldrich

来自 菜豆的凝集素 (红芸豆)

Erythroagglutinin PHA-E

别名:

PHA, 植物血球凝集素(菜豆)

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About This Item

UNSPSC代码:
12352202
NACRES:
NA.32

质量水平

形式

salt-free, lyophilized powder

效能

<10 μg per mL agglutination activity (with erythryocytes)

组成

Protein, ≥80% biuret

储存温度

2-8°C

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生化/生理作用

PHA由两种分子种类组成,即具有低促有丝分裂活性和高红细胞凝集素活性的红细胞凝集素(PHA-E),和具有高促有丝分裂和白细胞凝集活性但具有非常低的红细胞凝集活性的白细胞凝集素(PHA-L)。
PHA-E不具有血型特异性,但其凝集活性可被某些低聚糖抑制。 在分离和纯化红细胞凝集素和白细胞凝集素之前,PHA-P是PHA的蛋白质形式。 PHA-M是粘蛋白形式。 结合物由相应的纯化凝集素进行制备。

质量

检测白细胞凝集。

制备说明

通过改良 Leavitt 方法纯化。

分析说明

凝集活性以 μg/ml 表示,通过 1 mg/mL 溶液的系列稀释液测定。该活性是在 25℃ 下孵育 1 h 后凝集人红细胞(2% 磷酸盐缓冲盐水中,pH 6.8)或人白细胞(10 7 /mL 盐水中)混悬液的最低浓度。

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

动植物源性产品

分析证书(COA)

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Tuo Yang et al.
Cell reports, 29(9), 2917-2928 (2019-11-28)
Increased tolerance of Plasmodium falciparum to front-line artemisinin antimalarials (ARTs) is associated with mutations in Kelch13 (K13), although the precise role of K13 remains unclear. Here, we show that K13 mutations result in decreased expression of this protein, while mislocalization
R D Leavitt et al.
The Journal of biological chemistry, 252(9), 2961-2966 (1977-05-10)
Affinity-purified phytohemagglutinin from red kidney bean resolves into five isolectins by SP-Sephadex ion exchange chromatography. Recoveries ranging from 30 to 130 mg of protein for each isolectin are easily achieved. The isolectins have similar amino acid compositions which differ only
Malvina Prapa et al.
NPJ precision oncology, 5(1), 93-93 (2021-10-29)
Glioblastoma is the most malignant primary brain tumor and is still in need of effective medical treatment. We isolated patient-derived glioblastoma cells showing high GD2 antigen expression representing a potential target for CAR T strategy. Data highlighted a robust GD2
H J Hoffmann et al.
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 33(7), 930-935 (2003-07-16)
Atopy is closely associated with the cellular T helper type-2 (Th2) phenotype, that is dominated by the pleiotrophic cytokine IL-4. The cellular source of IL-4 has yet to be determined, although basophils have been proposed. Eosinophils and mast cells are

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