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应用
来自费氏弧菌的荧光素酶已用于一项评估生物发光细菌的光发射和氧消耗动力学的研究中。它也被用于一项调查各种发光细菌的暗突变体对活性氧物种敏感性的研究中。
特点和优势
部分纯化的、含有FMN依赖性荧光素酶和NADH-和NADPH依赖性FMN还原酶的可溶性提取物。在含有FMN、NADH或NADPH和正癸基醛的体系中产生光。
其他说明
ATCC No. 7744平衡主要的缓冲液和稳定剂。
外形
部分纯化的冻干粉
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
从最新的版本中选择一种：
分析证书(COA)
Lot/Batch Number
J J Bourgois et al.
Journal of bioenergetics and biomembranes, 33(4), 353-363 (2001-11-17)
Oxygen plays a key role in bacterial bioluminescence. The simultaneous and continuous kinetics of oxygen consumption and light emission during a complete exhaustion of the exogenous oxygen present in a closed system has been investigated. The kinetics are performed with
Patricia G Cruz et al.
Chemistry & biology, 18(11), 1442-1452 (2011-11-29)
The chemical diversity of nature has tremendous potential for the discovery of molecular probes and medicinal agents. However, sensitivity of HTS assays to interfering components of crude extracts derived from plants, and macro- and microorganisms has curtailed their use in
Loredana Peca et al.
FEMS microbiology letters, 289(2), 258-264 (2008-11-20)
Two whole-cell bioluminescent reporters were constructed by fusing the reporter genes luxAB with the Co(2+) and Zn(2+) inducible coaT promoter or the Ni(2+)-inducible nrsBACD promoter, respectively, in the genome of Synechocystis sp. PCC 6803. The obtained reporters, designated coaLux and
Anya Bakhrat et al.
Cell biology and toxicology, 27(3), 227-236 (2011-03-03)
We describe a Saccharomyces cerevisiae bioluminescence assay for UV and arsenate in which bacterial luciferase genes are regulated by the promoter of the yeast gene, UFO1. UFO1 encodes the F-box subunit of the Skp1–Cdc53–F-box protein ubiquitin ligase complex and is
Xin-Xin Huang et al.
Huan jing ke xue= Huanjing kexue, 29(11), 3159-3165 (2009-02-04)
Recombinate luminescence bacteria have the important role in evaluating water toxicity. Two recombinate luminescence bacteria vectors PUCD-uvrA and PUCD-alkA were constructed to investigate the impaired mechanism of pollutant genetic toxicity. The genes of uvrA and alkA were amplified by PCR
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