L8507
荧光素酶 来源于费希尔(氏)弧菌 （发光菌属 f）
lyophilized powder
别名:
细菌荧光素酶, 荧光素酶 来源于费氏发光杆菌
产品名称
荧光素酶 来源于费希尔(氏)弧菌 （发光菌属 f）, lyophilized powder
form
lyophilized powder
composition
Protein, ~40% biuret
storage temp.
−20°C
Quality Level
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Application
来自费氏弧菌的荧光素酶已用于一项评估生物发光细菌的光发射和氧消耗动力学的研究中。它也被用于一项调查各种发光细菌的暗突变体对活性氧物种敏感性的研究中。
Features and Benefits
部分纯化的、含有FMN依赖性荧光素酶和NADH-和NADPH依赖性FMN还原酶的可溶性提取物。在含有FMN、NADH或NADPH和正癸基醛的体系中产生光。
Other Notes
ATCC No. 7744平衡主要的缓冲液和稳定剂。
Physical form
部分纯化的冻干粉
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
J J Bourgois et al.
Journal of bioenergetics and biomembranes, 33(4), 353-363 (2001-11-17)
Oxygen plays a key role in bacterial bioluminescence. The simultaneous and continuous kinetics of oxygen consumption and light emission during a complete exhaustion of the exogenous oxygen present in a closed system has been investigated. The kinetics are performed with
Patricia G Cruz et al.
Chemistry & biology, 18(11), 1442-1452 (2011-11-29)
The chemical diversity of nature has tremendous potential for the discovery of molecular probes and medicinal agents. However, sensitivity of HTS assays to interfering components of crude extracts derived from plants, and macro- and microorganisms has curtailed their use in
E N Esimbekova et al.
Biochemistry. Biokhimiia, 74(6), 695-700 (2009-08-04)
The properties of a coupled enzyme system (NAD(P)H:FMN-oxidoreductase and luciferase) from luminous bacteria were studied. The enzymes and their substrates were immobilized in polymer gels of different types: starch (polysaccharide) and gelatin (polypeptide). Maximum activity yield (100%) was achieved with
M-L Foucault et al.
Applied and environmental microbiology, 76(1), 264-274 (2009-11-03)
Bioluminescence imaging (BLI) is emerging as a powerful tool for real-time monitoring of infections in living animals. However, since luciferases are oxygenases, it has been suggested that the requirement for oxygen may limit the use of BLI in anaerobic environments
Ismael Rodea-Palomares et al.
Archives of environmental contamination and toxicology, 57(3), 477-487 (2009-01-27)
The bioavailability and therefore toxicity of a metal depends on the chemical species present in a particular environment. We evaluated the effect of a series of factors that could potentially modify metal speciation on the toxicity of Hg, Cu, Zn
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