Application
来自费氏弧菌的荧光素酶已用于一项评估生物发光细菌的光发射和氧消耗动力学的研究中。它也被用于一项调查各种发光细菌的暗突变体对活性氧物种敏感性的研究中。
Features and Benefits
部分纯化的、含有FMN依赖性荧光素酶和NADH-和NADPH依赖性FMN还原酶的可溶性提取物。在含有FMN、NADH或NADPH和正癸基醛的体系中产生光。
Physical form
部分纯化的冻干粉
Other Notes
ATCC No. 7744平衡主要的缓冲液和稳定剂。
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
存储类别
11 - Combustible Solids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
Patricia G Cruz et al.
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The chemical diversity of nature has tremendous potential for the discovery of molecular probes and medicinal agents. However, sensitivity of HTS assays to interfering components of crude extracts derived from plants, and macro- and microorganisms has curtailed their use in
Loredana Peca et al.
FEMS microbiology letters, 289(2), 258-264 (2008-11-20)
Two whole-cell bioluminescent reporters were constructed by fusing the reporter genes luxAB with the Co(2+) and Zn(2+) inducible coaT promoter or the Ni(2+)-inducible nrsBACD promoter, respectively, in the genome of Synechocystis sp. PCC 6803. The obtained reporters, designated coaLux and
Thomas E Crowley
Biochemistry and molecular biology education : a bimonthly publication of the International Union of Biochemistry and Molecular Biology, 39(2), 126-132 (2011-03-30)
The genes responsible for luminescence in various species of the marine microorganism Photobacterium, have been used for many years as a tool by researchers and instructors. In particular, the lux operon of Photobacterium fischeri has been used by many instructors
Xin-Xin Huang et al.
Huan jing ke xue= Huanjing kexue, 29(11), 3159-3165 (2009-02-04)
Recombinate luminescence bacteria have the important role in evaluating water toxicity. Two recombinate luminescence bacteria vectors PUCD-uvrA and PUCD-alkA were constructed to investigate the impaired mechanism of pollutant genetic toxicity. The genes of uvrA and alkA were amplified by PCR
Elena V Vetrova et al.
Biophysical chemistry, 141(1), 59-65 (2009-01-24)
The interaction of quinone with luciferase from Photobacterium leiognathi was studied based on the fluorescence decay measurements of the endogenous flavin bound to the enzyme. Homologous 1,4-quinones, 1,4-benzoquinone, methyl-1,4-benzoquinone, 2-methyl-5-isopropyl-1,4-benzoquine and 1,4-naphthoquinone, were investigated. In the absence of quinone, the
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