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Merck
CN

L7647

Sigma-Aldrich

伴刀豆球蛋白A 来源于洋刀豆 (刀豆)

Type VI, lyophilized powder

别名:

刀豆素 A

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About This Item

CAS号:
EC 号:
MDL编号:
UNSPSC代码:
12352202
NACRES:
NA.32

生物来源

Canavalia ensiformis

质量水平

类型

Type VI

形式

lyophilized powder

效能

≤20 μg per mL agglutination activity

杂质

Carbohydrate, essentially free
salt, essentially free

溶解性

water: 1 % (w/v)

储存温度

−20°C

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应用

来自洋刀豆(洋刀豆)的伴刀豆球蛋白 A,用于开发两性离子固定化分子印迹聚合物(ziMIPs),用于蛋白质结晶。它还用于制备载有蛋白质的微凝胶,以防止微凝胶的酶促降解,通过共聚焦激光扫描显微镜(CLSM)进行监测。

生化/生理作用

刀豆素 A 无血型特异性,但对末端 α-D-甘露糖基和 α-D-葡糖基残基具有亲和力。需要 Ca2+ 和 Mn2+ 离子存在才能显示活性。刀豆素 A 在 pH 6.5 或更低的条件下解离为二聚体。pH 在 5.8 至 7.0 之间时,刀豆素 A 以四聚体形式存在;pH 高于 7.0 时,形成更高的聚合体。根据聚合程度不同,刀豆素 A 可能显示出具有促有丝分裂的活性。琥珀酰化可以生成一种能够在 pH 高于 5.6 时仍保持二聚体形式的活性二聚体。

制备说明

通过特异性亲和层析高度纯化。

分析说明

凝集活动:根据 1mg/mL 溶液的连续稀释液测定(该活性是在 25℃,温育 1 小时后,凝集 2% 人红细胞悬浮液的最低浓度)。 需要 A 型人血。

象形图

Health hazard

警示用语:

Danger

危险声明

危险分类

Repr. 2 - Resp. Sens. 1 - Skin Sens. 1

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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