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生物来源
Canavalia ensiformis
质量水平
类型
Type VI
表单
lyophilized powder
效能
≤20 μg per mL agglutination activity
杂质
Carbohydrate, essentially free
salt, essentially free
溶解性
water: 1 % (w/v)
储存温度
−20°C
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应用
来自洋刀豆（洋刀豆）的伴刀豆球蛋白 A，用于开发两性离子固定化分子印迹聚合物（ziMIPs），用于蛋白质结晶。它还用于制备载有蛋白质的微凝胶，以防止微凝胶的酶促降解，通过共聚焦激光扫描显微镜（CLSM）进行监测。
生化/生理作用
刀豆素 A 无血型特异性，但对末端 α-D-甘露糖基和 α-D-葡糖基残基具有亲和力。需要 Ca2+ 和 Mn2+ 离子存在才能显示活性。刀豆素 A 在 pH 6.5 或更低的条件下解离为二聚体。pH 在 5.8 至 7.0 之间时，刀豆素 A 以四聚体形式存在；pH 高于 7.0 时，形成更高的聚合体。根据聚合程度不同，刀豆素 A 可能显示出具有促有丝分裂的活性。琥珀酰化可以生成一种能够在 pH 高于 5.6 时仍保持二聚体形式的活性二聚体。
制备说明
通过特异性亲和层析高度纯化。
分析说明
凝集活动：根据 1mg/mL 溶液的连续稀释液测定（该活性是在 25℃，温育 1 小时后，凝集 2％ 人红细胞悬浮液的最低浓度）。 需要 A 型人血。
警示用语：
Danger
危险声明
危险分类
Repr. 2 - Resp. Sens. 1 - Skin Sens. 1
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Marion Schiavone et al.
FEMS yeast research, 15(2) (2015-03-13)
The Saccharomyces cerevisiae cell surface is endowed with some relevant technological properties, notably antimicrobial and biosorption activities. For these purposes, yeasts are usually processed and packaged in an 'autolysed/dried' formula, which may have some impacts on cell surface properties. In
Zwitterion-Immobilized Imprinted Polymers for Promoting the Crystallization of Proteins
Xing Y, et al.
Crystal Growth & Design, 15(10), 4932-4937 (2015)
Sevan Arabaciyan et al.
Frontiers in cell and developmental biology, 9, 607628-607628 (2021-02-16)
Single-cell variability of growth is a biological phenomenon that has attracted growing interest in recent years. Important progress has been made in the knowledge of the origin of cell-to-cell heterogeneity of growth, especially in microbial cells. To better understand the
Chain and pore-blocking effects on matrix degradation in protein-loaded microgels
Widenbring R, et al.
Biomacromolecules, 15(10), 3671-3678 (2014)
Lindsay Kosack et al.
Scientific reports, 7(1), 11289-11289 (2017-09-14)
Lipid metabolism is increasingly being appreciated to affect immunoregulation, inflammation and pathology. In this study we found that mice infected with lymphocytic choriomeningitis virus (LCMV) exhibit global perturbations of circulating serum lipids. Mice lacking the lipid-sensing surface receptor triggering receptor
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