应用
来自洋刀豆(洋刀豆)的伴刀豆球蛋白 A,用于开发两性离子固定化分子印迹聚合物(ziMIPs),用于蛋白质结晶。它还用于制备载有蛋白质的微凝胶,以防止微凝胶的酶促降解,通过共聚焦激光扫描显微镜(CLSM)进行监测。
生化/生理作用
刀豆素 A 无血型特异性,但对末端 α-D-甘露糖基和 α-D-葡糖基残基具有亲和力。需要 Ca2+ 和 Mn2+ 离子存在才能显示活性。刀豆素 A 在 pH 6.5 或更低的条件下解离为二聚体。pH 在 5.8 至 7.0 之间时,刀豆素 A 以四聚体形式存在;pH 高于 7.0 时,形成更高的聚合体。根据聚合程度不同,刀豆素 A 可能显示出具有促有丝分裂的活性。琥珀酰化可以生成一种能够在 pH 高于 5.6 时仍保持二聚体形式的活性二聚体。
制备说明
通过特异性亲和层析高度纯化。
分析说明
凝集活动:根据 1mg/mL 溶液的连续稀释液测定(该活性是在 25℃,温育 1 小时后,凝集 2% 人红细胞悬浮液的最低浓度)。 需要 A 型人血。
警示用语:
Danger
危险声明
危险分类
Repr. 2 - Resp. Sens. 1 - Skin Sens. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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