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Merck
CN

L7381

Sigma-Aldrich

花生的凝集素

FITC conjugate, lyophilized powder

别名:

PNA, 花生凝集素

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About This Item

UNSPSC代码:
12352202
NACRES:
NA.32

偶联物

FITC conjugate

质量水平

表单

lyophilized powder

组成

Protein, ~10% Lowry

标记范围

4-8 mol FITC per mol protein

储存温度

−20°C

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一般描述

凝集素是碳水化合物结合蛋白,广泛存在于真菌、植物和动物中。植物和动物凝集素的结构经过了各种不同研究。该蛋白的二级结构富含β折叠,表面具有糖类结合位点。

应用

花生凝集素已用于:
  • 测定活性精子细胞的顶体状态(存在或不存在顶体基质,对应完整或已经过顶体反应的精子)
  • 荧光染料标记PNA和B220抗体探测纵隔淋巴结(mLN)冷冻切片以检测生发中心(GC)
  • 可视化预处理视网膜切片的锥体外节

生化/生理作用

凝集素可用于糖缀合物的表征、成像和靶向研究。它用于微阵列分析,可实现高效的糖组谱分析,因其特异性作用于寡糖、糖蛋白和糖脂。植物和真菌中的凝集素可抵抗病原体/害虫(feeder)侵袭。凝集素参与宿主识别和组织粘附,从而有助于微生物致病机能。
花生凝集素不会凝集正常的人类红细胞,但会强烈凝集神经氨酸酶处理的红细胞。花生凝集素具有与人血清中的抗 T 抗体相似的有效抗 T 活性。凝集素可用于区分人淋巴细胞亚群。

外形

含有磷酸盐缓冲盐和 NaCl

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

动植物源性产品

从最新的版本中选择一种:

分析证书(COA)

Lot/Batch Number

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  1. How does the storage temperature relate to shipping conditions?

    The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.

  2. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  3. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  4. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  5. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  6. Is the peanut lectin (Lectin from Arachis hypogaea) a glycoprotein?  If so, what is the carbohydrate content?

    Peanut lectin is not a glycoprotein. Although peanut lectin binds specifically to a particular carbohydrate (beta-gal(1-->3)galNac) in other molecules, the lectin itself does not contain any carbohydrate.  Please see page 231 of the following paper: R. Banerjee, et al., "Crystal structure of peanut lectin, a protein with an unusual quaternary structure".  Proc. Nat. Acad. Sci., USA, 91, 227-231 (1994).

  7. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

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