产品名称
来自 荆豆(gorse、furze)的凝集素, lyophilized powder
form
lyophilized powder
potency
<8 μg/mL agglutination activity
composition
Protein, ≥80% biuret
impurities
salt, essentially free
storage temp.
2-8°C
Quality Level
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Analysis Note
凝集活性以μg/ml表示,由磷酸盐缓冲盐水(pH 6.8)连续稀释1 mg/ml溶液而测得。该活性是指25℃孵育1小时后使2%人O型血红细胞悬浮液凝集的最低浓度。
Biochem/physiol Actions
荆豆凝集素具有抗H血型特异性。凝集素有两种类型: UEA I 对 L-岩藻糖具有亲和力,而 UEA II 对 N,N′-二乙酰壳二糖具有亲和力。最初认为UEA I摩尔量为170,000道尔顿。 后来的报道表明,UEA I可以在中性和碱性pH下形成聚集体,正确的摩尔量是68,000道尔顿。
Other Notes
由亲和纯化的凝集素制备缀合物。
Preparation Note
使用Pereira的方法通过亲和色谱法纯化。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
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Immunochemical studies on the combining site of the blood group H-specific lectin 1 from Ulex europeus seeds.
M E Pereira et al.
Archives of biochemistry and biophysics, 185(1), 108-115 (1978-01-15)
D Guy et al.
British journal of cancer, 42(6), 915-921 (1980-12-01)
Present evidence suggests that the cell surface has an important role in metastasis. To examine this idea further, the surface properties of single cells isolated from the primary growths of a liver-metastasizing (ML) and a non-metastasizing (NML) lymphosarcoma were compared
R T Sawyer et al.
Infection and immunity, 60(3), 1041-1046 (1992-03-01)
The isolated perfused mouse liver model was used to study the effects of various lectins on hepatic trapping and killing of Candida albicans. After mouse livers were washed with 20 to 30 ml of perfusion buffer, 10(6) C. albicans CFU
M Kristensen et al.
Scandinavian journal of medicine & science in sports, 25 Suppl 2, 40-48 (2015-06-18)
We investigated the effect of alpine skiing for 12 weeks on skeletal muscle characteristics and biomarkers of glucose homeostasis and cardiovascular risk factors. Twenty-three patients with a total knee arthroplasty (TKA) were studied 2.9 ± 0.9 years (mean ± SD) after the operation. Fourteen
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