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生物来源
Bandieraea simplicifolia
质量水平
偶联物
peroxidase conjugate
方案
≥90% protein basis (Warburg-Christian)
表单
lyophilized powder
效能
<200 μg per mL agglutination activity (using human blood group B erythrocytes)
peroxidase activity
40-160 units/mg protein
组成
Protein, ~95% modified Warburg-Christian
技术
blood typing: suitable
fractionation: suitable
储存温度
−20°C
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相关类别
应用
通用蛋白质印迹方案：
- 糖蛋白样品规格：500ng
- 凝集素浓度： 0.1ug/ml
- 每条泳道上样500 ng糖蛋白样品
- 进行4-20% Bis-Tris SDS page凝胶
- 转膜凝胶至PVDF膜上
- 用于RIPA缓冲液（R0278 Sigma）在室温对膜进行封闭1小时
- 在室温用RIPA缓冲液以0.1 ug/ml孵育HRP凝集素2小时
- 用25ml RIPA缓冲液洗膜 5 x 5分钟
- 用化学发光底物（CPS1-120）进行检测
生化/生理作用
BS-I对末端α-D-半乳糖残基具有主要亲和力，对末端N-乙酰基-α-D-半乳糖胺残基具有二级亲和力。
其他说明
BS-I是一种四聚体凝集素，由两种类型的亚基A和B组成。存在由不同亚基组成的五种BS-I同工凝集素：BSI-B4、BSI-AB3、BSI-A2B2、BSI-A3B、以及BSI-A4。BSI-B4 是血型B特异的，其对末端α-D-半乳糖残基具有独特的亲和力，而BSI-A4 具有血型A特异性，其对末端N-乙酰基-α-D-半乳糖胺残基具有主要亲和力。
单位定义
在20℃、pH值为6.0条件下，1个单位的连苯三酚在20 s内会形成1 mg红棓酚。
外形
含柠檬酸钠缓冲盐和氯化钙
制备说明
使用O′Sullivan等人的方法的改进，由VI型过氧化物酶制备。偶联后通过亲和层析重新纯化。
分析说明
凝集活性以 μg/mL表示，并且使用磷酸盐缓冲盐水（pH6.8）对1mg/mL溶液进行连续稀释液来测定，对于每种凝集素，所含有钙、镁和锰的浓度不同。该活性是在25℃孵育1小时后，对2％合适的红细胞悬浮液进行凝集所需的最低浓度。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Hélène Fe Gleitz et al.
EMBO molecular medicine, 10(7) (2018-06-10)
The pediatric lysosomal storage disorder mucopolysaccharidosis type II is caused by mutations in IDS, resulting in accumulation of heparan and dermatan sulfate, causing severe neurodegeneration, skeletal disease, and cardiorespiratory disease. Most patients manifest with cognitive symptoms, which cannot be treated
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Scientific reports, 10(1), 16223-16223 (2020-10-03)
Stereotactic radiosurgery (SRS) has proven an effective tool for the treatment of brain tumors, arteriovenous malformation, and functional conditions. However, radiation-induced therapeutic effect in viable cells in functional SRS is also suggested. Evaluation of the proposed modulatory effect of irradiation
Christophe Dubois et al.
Journal of the American College of Cardiology, 55(20), 2232-2243 (2010-05-15)
We compared biological repair after acute myocardial infarction (AMI) with selected porcine progenitor cell populations. Cell types and mechanisms responsible for myocardial repair after AMI remain uncertain. In a blinded, randomized study, we infused autologous late-outgrowth endothelial progenitor cells (EPC)
Qing Wang et al.
Neurobiology of disease, 36(1), 223-231 (2009-07-29)
Pyruvate, a key intermediate in glucose metabolism, was explored as a potential treatment in models of experimental stroke and inflammation. Pyruvate was administered to rodents after the onset of middle cerebral artery occlusion (MCAO). Since the extent of inflammation is
Inflammation and NFkappaB activation is decreased by hypothermia following global cerebral ischemia.
Carla M Webster et al.
Neurobiology of disease, 33(2), 301-312 (2008-12-10)
We previously showed that hypothermia attenuates inflammation in focal cerebral ischemia (FCI) by suppressing activating kinases of nuclear factor-kappa B (NFkappaB). Here we characterize the inflammatory response in global cerebral ischemia (GCI), and the influence of mild hypothermia. Rodents were
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