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生物来源
Bandieraea simplicifolia
质量水平
偶联物
peroxidase conjugate
检测方案
≥90% protein basis (Warburg-Christian)
形式
lyophilized powder
效能
<200 μg per mL agglutination activity (using human blood group B erythrocytes)
peroxidase activity
40-160 units/mg protein
组成
Protein, ~95% modified Warburg-Christian
技术
blood typing: suitable
fractionation: suitable
储存温度
−20°C
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相关类别
应用
通用蛋白质印迹方案：
- 糖蛋白样品规格：500ng
- 凝集素浓度： 0.1ug/ml
- 每条泳道上样500 ng糖蛋白样品
- 进行4-20% Bis-Tris SDS page凝胶
- 转膜凝胶至PVDF膜上
- 用于RIPA缓冲液（R0278 Sigma）在室温对膜进行封闭1小时
- 在室温用RIPA缓冲液以0.1 ug/ml孵育HRP凝集素2小时
- 用25ml RIPA缓冲液洗膜 5 x 5分钟
- 用化学发光底物（CPS1-120）进行检测
生化/生理作用
BS-I对末端α-D-半乳糖残基具有主要亲和力，对末端N-乙酰基-α-D-半乳糖胺残基具有二级亲和力。
其他说明
BS-I是一种四聚体凝集素，由两种类型的亚基A和B组成。存在由不同亚基组成的五种BS-I同工凝集素：BSI-B4、BSI-AB3、BSI-A2B2、BSI-A3B、以及BSI-A4。BSI-B4 是血型B特异的，其对末端α-D-半乳糖残基具有独特的亲和力，而BSI-A4 具有血型A特异性，其对末端N-乙酰基-α-D-半乳糖胺残基具有主要亲和力。
单位定义
在20℃、pH值为6.0条件下，1个单位的连苯三酚在20 s内会形成1 mg红棓酚。
外形
含柠檬酸钠缓冲盐和氯化钙
制备说明
使用O′Sullivan等人的方法的改进，由VI型过氧化物酶制备。偶联后通过亲和层析重新纯化。
分析说明
凝集活性以 μg/mL表示，并且使用磷酸盐缓冲盐水（pH6.8）对1mg/mL溶液进行连续稀释液来测定，对于每种凝集素，所含有钙、镁和锰的浓度不同。该活性是在25℃孵育1小时后，对2％合适的红细胞悬浮液进行凝集所需的最低浓度。
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
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