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生物来源
bovine milk
质量水平
类型
Type I
方案
≥85% (PAGE)
表单
lyophilized powder
技术
cell culture | mammalian: suitable
electrophoresis: suitable
溶解性
H2O: soluble 10 mg/mL, clear to slightly hazy, colorless to faintly yellow
UniProt登记号
储存温度
−20°C
基因信息
cow ... LALBA(281894)
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一般描述
α-乳清蛋白是一种分子量较小的球状乳清蛋白,存在于迄今为止已研究的所有乳汁中。它是在乳腺中产生的约14kDa的金属蛋白。
应用
α-从牛乳中提取的乳清蛋白作为补充剂,用于各种细胞培养的基础培养基。也已作为标记物,用于十二烷基硫酸钠-聚丙烯酰胺凝胶电泳(SDS-PAGE)。
生化/生理作用
α-乳清蛋白是人乳汁中的主要蛋白。它由单个多肽链组成,含有通过二硫键连接的8个半胱氨酸。α-乳清蛋白可结合多种金属离子,包括钙,这一性质被认为在从还原的变性形式再生成天然α-乳清蛋白的过程中发挥重要作用。α-乳清蛋白还具有独特的锌结合位点,其被认为在乳糖合酶复合物的结合中发挥作用。 成熟蛋白质由123个氨基酸残基(14kD)组成,并且具有1.7 Α°分辨率三维结构,含四个α-螺旋和三链反式平行β-折叠。
其可通过改变半乳糖基转移酶的底物特异性以增加乳糖形成的速率;半乳糖基转移酶和 α-乳白蛋白的复合物称为乳糖合酶。
其可通过改变半乳糖基转移酶的底物特异性以增加乳糖形成的速率;半乳糖基转移酶和α-乳白蛋白的复合物称为乳糖合酶。Asp87 或 Asp88 对 Ala 的定点诱变完全消除了强钙结合亲和力,并将乳糖合酶的刺激降低到最大速率的 <3.5%。
质量
钙饱和的。可能含有微量的硫酸铵和磷酸钠
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
历史批次信息供参考:
分析证书(COA)
Journal of colloid and interface science, 344(1), 54-60 (2010-01-23)
We recently showed the possibility to build dopamine-melanin films of controlled thickness by successive immersions of a substrate in alkaline solutions of dopamine [F. Bernsmann, A. Ponche, C. Ringwald, J. Hemmerlé, J. Raya, B. Bechinger, J.-C. Voegel, P. Schaaf, V.
Effect of different exogenous fatty acids on the cytosolic triacylglycerol content in bovine mammary cells
Animal Nutrition, 5(2), 202-208 (2019)
Food science & nutrition, 9(4), 2299-2307 (2021-04-13)
α-Dicarbonyl compounds (α-DCs) are a class of compounds generated during the thermal processing of food. Due to the high reactivity, α-DCs were endowed with the ability to react with food components thus lowering nutrition value and even leading to a
Soft matter, 15(24), 4787-4796 (2019-05-08)
Formation of nanotubes from partially hydrolysed α-lactalbumin (α-La) was investigated at five pH values, two concentrations of α-La and two calcium levels. Nanotubes were formed under almost all combinations of the investigated factors, and for the first time the formation
The Journal of nutrition, 140(2), 382-391 (2009-12-25)
Innate immunity depends on the efficiency of neutrophils to be activated rapidly to restore homeostasis. It can benefit from priming agents that enhance neutrophil capacity to respond more efficiently to a subsequent stimulation. Among natural products, a bovine whey protein
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