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一般描述
来自Triticum vulgaris的小麦胚芽凝集素（WGA）以同型二聚体形式存在，并且具有多个N-乙酰葡糖胺（GlcNAc）结合位点。它对蛋白质变性剂具有抗性，可用于检测 O-GlcNAc蛋白。 WGA与N-乙醇酰神经氨酸（Neu5Ac）结合可调节促炎免疫应答。
应用
来自Triticum vulgaris （小麦）的凝集素已被用于：
- 丝虫寄生虫的子宫内阶段的样品的凝集素结合测定中
- 小鼠心肌细胞的组织学研究中
- 与Dynabeads一起形成珠子-生物素-WGA复合物® M-280链霉抗生物素蛋白磁珠，用于对来自水样中的弓形虫卵母细胞中的凝集素进行磁性分离
生化/生理作用
WGA 不具有血型特异性，但具有与 N-乙酰基-β-D-葡糖胺残基和 N-乙酰基-β-D-葡糖胺低聚物的亲和性。WGA 不含蛋白结合糖。
包装
包装尺寸基于蛋白质含量。
外形
含有柠檬酸钠
制备说明
由亲和纯化凝集素制备偶联物。
法律信息
Dynabeads is a registered trademark of Invitrogen Dynal AS Corp
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
WGA-based lectin affinity gel electrophoresis: A novel method for the detection of O-GlcNAc-modified proteins
Kubota Y, et al.
Testing, 12(7), e0180714-e0180714 (2017)
The dietary intake of wheat and other cereal grains and their role in inflammation
De PK and Pruimboom L
Nutrients, 5(3), 771-787 (2013)
R E Morris et al.
The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society, 40(5), 711-721 (1992-05-01)
We demonstrate here that the intracellular routing of biotinylated ligands was not affected by the attachment of streptavidin gold colloids so long as the electron-dense marker was added after the biotinyl ligand-receptor interaction had occurred. The binding, internalization, and intracellular
Candida Fagundes Teixeira et al.
PLoS neglected tropical diseases, 1(2), e73-e73 (2007-12-07)
Diagnosis of intestinal schistosomiasis in low endemic areas is a problem because often control measures have reduced egg burdens in feces to below the detection limits of classical coproparasitological methods. Evaluation of molecular methods is hindered by the absence of
Evaluation of bi-ventricular coronary flow patterns using high-frequency ultrasound in mice with transverse aortic constriction
Wu J, et al.
Ultrasound in Medicine & Biology, 39(11), 2053-2065 (2013)
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