L4418
Anti-LSD1 (AOF2) (C-terminal) antibody produced in rabbit
~1 mg/mL, affinity isolated antibody, buffered aqueous solution
别名:
Anti-Amine oxidase, flavin-containing, 2, Anti-BRAF-HDAC complex protein 110, Anti-KIAA0601 protein, Anti-Lysine-specific demethylase 1, Anti-NPAO, Anti-Nuclear Polyamine Oxidase
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所有图片(3)
About This Item
推荐产品
生物来源
rabbit
质量水平
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
分子量
antigen 110-115 kDa
种属反应性
human, mouse (predicted), rat (predicted)
浓度
~1 mg/mL
技术
immunocytochemistry: 5-10 μg/mL using MCF7 cells fixed with formaldehyde
immunoprecipitation (IP): 1-2 μg using MCF7 cell lysates
western blot: 1-2 μg/mL using MCF7 cell lysates (2 bands, 2 isoforms)
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... AOF2(23028)
mouse ... Aof2(99982)
一般描述
Lysine specific demethylase 1 (LSD1) also known as amine oxidase flavin containing domain 2 (AOF2) is a lysine specific demethylase. It is a nuclear protein containing a Swi3p, Rsc8p and Moira (SWIRM) domain, a flavin adenine dinucleotide (FAD)-binding motif and an amine oxidase domain.
免疫原
synthetic peptide corresponding to amino acids 835-852 of human LSD1 (AOF2) (isoform b), conjugated to KLH via an N-terminal added cysteine reisidue. The immunizing sequence is present also in isoform a. This sequence is conserved in human, rat, and mouse.
应用
Anti-LSD1 (AOF2) (C-terminal) antibody produced in rabbit immunoblotting, immunoprecipitation and immunofluorescence.
生化/生理作用
Lysine specific demethylase 1 (LSD1) is a component of several histone deacetylase complexes that function through modifying chromatin structure to repress transcription. LSD1 (AOF2) action can signal either activation or repression of transcription. Demethylation of histone H3 Lys4 by LSD1 (AOF2) leads to transcriptional repression of target genes, while demethylation of histone H3 at Lys9 leads to de-repression of androgen receptor target genes. The activity of LSD1 (AOF2) is regulated by its associated factors. REST corepressor 1 (CoREST), a transcriptional corepressor, promotes demethylation by enhancing the association of LSD1 (AOF2) with the nucleosomes, and it also protects LSD1 (AOF2) from proteasomal degradation.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
从最新的版本中选择一种：
分析证书(COA)
Lot/Batch Number
Regulation of LSD1 histone demethylase activity by its associated factors
Shi YJ, et al.
Molecular Cell, 19(6), 857-864 (2005)
Lysine-specific demethylase 1 regulates the embryonic transcriptome and CoREST stability
Foster CT, et al.
Molecular and Cellular Biology, 30(20), 4851-4863 (2010)
LSD1 demethylates repressive histone marks to promote androgen-receptor-dependent transcription
Metzger E, et al.
Nature, 437(7057), 436-436 (2005)
Histone demethylation mediated by the nuclear amine oxidase homolog LSD1
Shi Y, et al.
Cell, 119(7), 941-953 (2004)
Inosha D Gomes et al.
Chembiochem : a European journal of chemical biology, 20(11), 1444-1449 (2019-02-01)
Histone deacetylase 1 (HDAC1) regulates transcription by deacetylating histones. In addition to histones, several non-histone proteins are HDAC1 substrates, which suggests a role for HDAC1 beyond epigenetics. Unfortunately, the identification of non-histone substrates has been largely serendipitous, which makes full characterization
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