生物来源
Triticum vulgaris
质量水平
偶联物
peroxidase conjugate
方案
≥85% protein basis (Warburg-Christian)
表单
lyophilized powder
效能
<40 μg/mL agglutination activity
peroxidase activity
50-200 units/mg protein
组成
Protein, ~90% modified Warburg-Christian
储存温度
−20°C
正在寻找类似产品? 访问 产品对比指南
应用
用适当的过氧化物酶底物检测含有β(1→4)-N-乙酰基-D-葡糖胺的糖蛋白
通用Western Blot实验方法：
- 糖蛋白样品规格：500ng
- 凝集素浓度：0.1ug/ml
- 每条泳道上样500 ng糖蛋白样品
- 进行4-20% Bis-Tris SDS page凝胶
- 转膜至PVDF膜上
- 用于RIPA缓冲液（R0278 Sigma）在室温对膜进行封闭1小时
- 在室温用RIPA缓冲液以0.1 ug/ml孵育HRP凝集素2小时
- 用25ml RIPA缓冲液洗膜 5 x 5分钟
- 用化学发光底物（CPS1-120）进行检测
生化/生理作用
WGA 不具有血型特异性，但具有与 N-乙酰基-β-D-葡糖胺残基和 N-乙酰基-β-D-葡糖胺低聚物的亲和性。WGA 不含蛋白结合糖。
包装
包装尺寸基于蛋白质含量。
外形
含有柠檬酸盐缓冲液
制备说明
由亲和纯化凝集素制备偶联物。
通过改良的O′Sullivan方法，使用VI型过氧化物酶（P8375）而制备，对低分子量耦合物具有倾向性。在耦合后通过亲和层析进行再纯化。
其他说明
在20℃、pH 6.0条件下，一单位可在20秒内从邻苯三酚形成1.0 mg的红棓酚。
储存分类代码
11 - Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
动植物源性产品
此项目有
Xinyu Qi et al.
Journal of bone and joint infection, 6(7), 241-253 (2021-07-16)
The high antibiotic tolerance of Staphylococcus aureus biofilms is associated with challenges for treating periprosthetic joint infection. The toxin-antitoxin system, YefM-YoeB, is thought to be a regulator for antibiotic tolerance, but its physiological role is unknown. The objective of this
Hugo Aguilar-Díaz et al.
PLoS neglected tropical diseases, 4(2), e607-e607 (2010-02-20)
Inhibition of encystment can be conceived as a potentially useful mechanism to block the transmission of Entamoeba histolytica under natural conditions. Unfortunately, amoeba encystment has not been achieved in vitro and drugs inhibiting the formation of cysts are not available.
Qian Guo et al.
Journal of bone and mineral research : the official journal of the American Society for Bone and Mineral Research, 36(10), 2065-2080 (2021-06-23)
ATP-citrate lyase (ACLY), generating most of the nucleocytosolic acetyl coenzyme A (acetyl-CoA) for histone acetylation, links cell metabolism to epigenetic regulation. Recent investigations demonstrated that ACLY activated by metabolic reprogramming played an essential role in both M1 and M2 macrophage
Qian Guo et al.
The Journal of biological chemistry, 101775-101775 (2022-03-09)
It's widely accepted that increasing mitochondrial respiration plays a pivotal role during osteoclastogenesis. Mitochondrial pyruvate carrier (MPC) is the key transporter that links glycolysis to mitochondrial respiration but little is known about its role during osteoclastogenesis. Our goal was to
Junlan Liu et al.
Communications biology, 4(1), 904-904 (2021-07-24)
Though a definitive link between small colony variants (SCVs) and implant-related staphylococcal infections has been well-established, the specific underlying mechanism remains an ill-explored field. The present study analyzes the role SCVs play in catheter infection by performing genomic and metabolic
商品
Understand sialic acid structure, function, signaling, and modifications. Easily find products for sialic acid research.
Find the right lectin for your research with our lectin selection guide, organized by lectin source/species, carbohydrate specificity, blood group specificity, and more.
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持