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文件

安全信息

L3772

Sigma-Aldrich

Leptin from mouse

≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder

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别名:
OB
CAS号:
181030-10-4
MDL编号:
MFCD01094206
UNSPSC代码:
51111800
NACRES:
NA.32

生物来源

mouse

质量水平

200

重组

expressed in E. coli

无菌性

non-sterile

检测方案

≥98% (SDS-PAGE)

形式

lyophilized powder

效能

≤1.0 ng/mL ED50

分子量

predicted mol wt ~16 kDa

储存条件

OK to freeze

技术

cell based assay: suitable

杂质

≤1.0 EU/μg endotoxin

UniProt登记号

P41160

运输

ambient

储存温度

−20°C

SMILES字符串

CC(C)CC(C(=NC(CCC(=N)O)C(=NCC(=NC(CO)C(=NC(CC(C)C)C(=NC(CCC(=N)O)C(=NC(CC(=O)O)C(=NC(CCSC)C(=NC(CC(C)C)C(=NC(CC1=CNC2=CC=CC=C21)C(=NC(CCC(=N)O)C(=NC(CC(C)C)C(=NC(CC(=O)O)C(=NC(CC(C)C)C(=NC(CO)C(=O)N3CCCC3C(=NCC(=NC(CS)C(=O)O)O)O)O)O)O)O)O)O)O)O)O)O)O)O)O)O)N

InChI

1S/C87H138N22O28S2/c1-41(2)27-48(88)72(121)97-50(18-21-65(89)112)73(122)93-36-68(115)95-61(38-110)84(133)104-54(28-42(3)4)77(126)98-52(20-23-67(91)114)75(124)106-59(33-70(117)118)82(131)100-53(24-26-139-11)76(125)102-55(29-43(5)6)78(127)105-58(32-46-35-92-49-16-13-12-15-47(46)49)81(130)99-51(19-22-66(90)113)74(123)101-56(30-44(7)8)79(128)107-60(34-71(119)120)83(132)103-57(31-45(9)10)80(129)108-62(39-111)86(135)109-25-14-17-64(109)85(134)94-37-69(116)96-63(40-138)87(136)137/h12-13,15-16,35,41-45,48,50-64,92,110-111,138H,14,17-34,36-40,88H2,1-11H3,(H2,89,112)(H2,90,113)(H2,91,114)(H,93,122)(H,94,134)(H,95,115)(H,96,116)(H,97,121)(H,98,126)(H,99,130)(H,100,131)(H,101,123)(H,102,125)(H,103,132)(H,104,133)(H,105,127)(H,106,124)(H,107,128)(H,108,129)(H,117,118)(H,119,120)(H,136,137)/t48-,50-,51-,52-,53-,54-,55-,56-,57-,58-,59-,60-,61-,62-,63-,64-/m0/s1

InChI key

NRYBAZVQPHGZNS-ZSOCWYAHSA-N

基因信息

mouse ... Lep(16846)

相关类别

一般描述

Research area: Cell signaling

Leptin (Ob) is a non-glycosylated polypeptide hormone. It is produced primarily in adipocytes and the placenta. The structure of leptin closely resembles members of the long-chain helical cytokine family. It is composed of four antiparallel α-helices interconnected by two long crossover links and one short loop. These structural elements are arranged in a left-handed helical bundle formation, resulting in a unique two-layer packing configuration.

应用

Leptin from mouse has been used:
  • to check the effect of leptin on ovulation for treating infertility in obese mice
  • to check the effect of leptin on MGARP (mitochondria-localized glutamic acid-rich protein) expression in testis and follicular granular cells of mice
  • to study the morphometrical changes in mouse testicular tissue upon long term exposure to leptin

生化/生理作用

Leptin is a hormone produced primarily in adipocytes, although leptin mRNA has also been identified in placenta and fetal tissues, gastric tissue and liver. Its primary site of action appears to be on neurons in the hypothalamus that are involved in regulating energy balance, appetite, and body weight. Leptin increases the production of nitric oxide in endothelial cells and stimulates angiogenesis in vitro and in vivo.
Human and mouse leptin share ~84% sequence identity.
Leptin, an essential cytokine, serves as a signaling molecule in the reproductive system. It modulates placental functions through autocrine or paracrine signaling. Particularly in the early stages of pregnancy, leptin plays a vital role in modulating key processes including cell proliferation, protein synthesis, invasion, and apoptosis within placental cells. Deficiency of leptin may lead to the development of severe obesity and abnormalities in hematopoiesis, immunity, angiogenesis, bone formation, blood pressure regulation and reproduction. Absence of leptin in mice causes severe obesity and hypothermia at temperature below thermoneutrality. It also controls feeding behavior. Leptin signaling is crucial for the bone formation.

外形

Lyophilized from a 0.2 μm filtered solution with 0.0045 mM NaHCO3

分析说明

The biological activity is measured by its ability to induce proliferation of leptin-dependent human OB-R transfected murine BAF3 cells.

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

法规信息

监管及禁止进口产品

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Yan Gao et al.
The Journal of clinical investigation, 125(9), 3681-3691 (2015-08-25)
Dietary iron supplementation is associated with increased appetite. Here, we investigated the effect of iron on the hormone leptin, which regulates food intake and energy homeostasis. Serum ferritin was negatively associated with serum leptin in a cohort of patients with
P Pallares et al.
Theriogenology, 73(8), 1088-1095 (2010-02-23)
The goal of the current was to elucidate if treatment with gonadotrophins and leptin can circumvent infertility in obese mice and to establish whether reproductive effects of leptin are influenced at the hypothalamus-hypophysis or ovarian level by using a leptin
Leptin action in normal and pathological pregnancies
Perez-Perez P, et al.
Journal of Cellular and Molecular Medicine, 22(2), 716-727 (2018)
Intracellular signalling pathways activated by leptin
Fruhbeck G
The Biochemical Journal, 393(Pt 1), 7-20 (2006)
Jordan T White et al.
Hormones and behavior, 66(1), 169-179 (2014-03-04)
This article is part of a Special Issue "Energy Balance". Natural populations display a variety of reproductive responses to environmental cues, but the underlying physiology that causes these responses is largely unknown. This study tested the hypothesis that heritable variation
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