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Merck
CN
所有图片(2)

文件

安全信息

L3768

Sigma-Aldrich

溶壁酶 来源于曲霉

powder

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别名:
Cellulase, Lysing Enzymes, Pectinase
UNSPSC代码:
12352204
NACRES:
NA.54

生物来源

Aspergillus niger

质量水平

形式

powder

技术

cell based assay: suitable

颜色

brown

适用性

suitable for cell lysis

应用

cell analysis

储存温度

2-8°C

一般描述

Lysing enzymes are also known as lytic enzymes or cell wall lytic enzymes.

应用

该裂解酶(来自曲霉)用于:

  • 粟酒裂殖酵母(Schizosaccharomyces pombe)细胞壁消化
  • 哈茨木霉(Trichoderma harzianum)菌丝体细胞壁裂解
Lysing Enzymes from Aspergillus sp. has been used to digest the cell walls to create pulse-field gel plugs.

生化/生理作用

Lysing enzymes are a group of enzymes that can break down the cell walls of microorganisms. These enzymes play a crucial role in various biological processes, including cell lysis, tissue dissociation, and protein extraction. Lysing enzymes are commonly used in research, biotechnology, and clinical applications. They are especially useful in DNA extraction, where they help to release genetic material from cells by breaking down the cell walls. This enables efficient isolation and purification of DNA for downstream analyses. Cellulases are a class of enzymes that degrade cellulose, a major component of plant cell walls, into simpler sugars. These sugars can be utilized as raw materials to produce biofuels, biobased chemicals, plastics, and various other materials. Pectinases are a group of enzymes that hydrolyze pectic substances found in microorganisms and higher plants. They can break down the pectin-containing compounds synthesized by plants and microbes.

质量

含有果胶酶和纤维素酶活性。

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

法规信息

常规特殊物品

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Translesion synthesis polymerases contribute to meiotic chromosome segregation and cohesin dynamics in Schizosaccharomycespombe
Mastro TL, et al.
Journal of Cell Science (2020)
Unleashing the Promise of Biotechnology to Help Heal, Fuel, and Feed the World
Greenwood JC
Journal of biological and chemical chronicles (2014)
Tara L Mastro et al.
Journal of cell science, 133(10) (2020-04-23)
Translesion synthesis polymerases (TLSPs) are non-essential error-prone enzymes that ensure cell survival by facilitating DNA replication in the presence of DNA damage. In addition to their role in bypassing lesions, TLSPs have been implicated in meiotic double-strand break repair in
Pectinase from Microorganisms and Its Industrial Applications
Haile S and Ayele A
TheScientificWorldJournal (2022)
New strains obtained after UV treatment and protoplast fusion of native Trichoderma harzianum: their biocontrol activity on Pyrenochaeta lycopersici
Besoain X A, et al.
Electronic journal of Biotechnology, 10(4), 604-617 (2007)

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