L2769
来自 菜豆的凝集素 （红芸豆）
Leucoagglutinin PHA-L, lyophilized powder
别名:
PHA, 植物血球凝集素(菜豆)
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关于此项目
NACRES:
NA.32
UNSPSC Code:
12352202
Form:
lyophilized powder
Biological source:
Phaseolus vulgaris
biological source
Phaseolus vulgaris
form
lyophilized powder
potency
erythroagglutination activity (Does not agglutinate a 2% suspension of human erythrocytes at <62.5 μg lectin/ml.), ≤1.0 μg/mL leucoagglutination activity, ≤5 μg/mL mitogenic activity
composition
Protein, ≥60% biuret
impurities
salt, free
storage temp.
2-8°C
Quality Level
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General description
菜豆源植物血凝素(PHA)是一种四聚体糖蛋白。它由两个分子量120 kDa的不同亚基组成。
Application
菜豆（红芸豆）凝集素已用于：
- 用作ELISPOT（酶联免疫斑点）测定的阳性对照
- 诱导体外T细胞分化过程的Th2极化
- 用作淋巴细胞增殖测定的对照物
Biochem/physiol Actions
PHA由两种分子种类组成，即具有低促有丝分裂活性和高红细胞凝集素活性的红细胞凝集素（PHA-E），和具有高促有丝分裂和白细胞凝集活性但具有非常低的红细胞凝集活性的白细胞凝集素（PHA-L）。
PHA-E不具有血型特异性，但其凝集活性可被某些低聚糖抑制。 在分离和纯化红细胞凝集素和白细胞凝集素之前，PHA-P是PHA的蛋白质形式。 PHA-M是粘蛋白形式。 结合物由相应的纯化凝集素进行制备。
PHA-E不具有血型特异性，但其凝集活性可被某些低聚糖抑制。 在分离和纯化红细胞凝集素和白细胞凝集素之前，PHA-P是PHA的蛋白质形式。 PHA-M是粘蛋白形式。 结合物由相应的纯化凝集素进行制备。
Preparation Note
其根据修改的Leavitt方法进行纯化。
Analysis Note
凝集活性以 μg/ml表示，并由1mg/ml溶液的连续稀释液进行测定。该活性是在25℃孵育1小时后，凝集人红细胞（2％磷酸盐缓冲盐水，pH6.8）或人白细胞（每毫升盐水中含107 个细胞）悬浮液的最低浓度。
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
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