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生物来源
Escherichia coli (K-235)
质量水平
表单
lyophilized powder
纯化方式
phenol extraction
杂质
<3% Protein (Lowry)
颜色
white to faint yellow
溶解性
water: soluble
运输
ambient
储存温度
2-8°C
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相关类别
一般描述
该产品通过苯酚提取自大肠杆菌血清型K235。源菌株为ATCC 13027。
应用
- Establishment of disseminated intravascular coagulation (DIC) model by a single iv administration of Escherichia coli-derived lipopolysaccharide (LPS) to cynomolgus monkeys and evaluation of its pathophysiological status.：大肠杆菌来源脂多糖(LPS)用于建立弥散性血管内凝血(DIC)食蟹猴模型，解析DIC的病理状态和可能的治疗措施(Minomo et al., 2017)。
- Different bacterial lipopolysaccharides as toxicants and stressors in the shrimp Palaemon elegans.：该文研究各种细菌LPS（包括大肠杆菌K-235）对长臂虾(Palaemon elegans)的毒性作用，促进了解海洋生物的LPS诱导应激反应(Lorenzon et al., 2002)。
生化/生理作用
脂多糖（LPS）位于膜的外层，并且在非包封的菌株中，其暴露于细胞表面。它们有助于外膜的完整性，并可保护细胞免受胆汁盐和亲脂性抗生素的作用。
制备说明
该产品可溶于水（5mg / ml）或细胞培养基（1mg / ml），产生浑浊的淡黄色溶液。当涡旋并升温至70-80 oC后，在盐水中可获得更高浓度的溶液（20mg/ml），但仍然是浑浊的溶液。脂多糖是一种在各种溶剂中都形成胶束的分子。在水和磷酸盐缓冲盐水中可观察到浑浊的溶液。有机溶剂无法使溶液变澄清。甲醇会产生含有漂浮物的混浊悬浮液，而水会产生均匀的混浊溶液。
警示用语：
Danger
危险声明
危险分类
Acute Tox. 2 Oral
储存分类代码
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Jay W Chaplin et al.
Journal of immunology (Baltimore, Md. : 1950), 187(8), 4199-4209 (2011-09-16)
CD180 is homologous to TLR4 and regulates TLR4 signaling, yet its function is unclear. We report that injection of anti-CD180 mAb into mice induced rapid Ig production of all classes and subclasses, with the exception of IgA and IgG2b, with
Jessica F Hoffman et al.
The Journal of neuroscience : the official journal of the Society for Neuroscience, 36(39), 10039-10049 (2016-09-30)
Identifying and understanding critical periods in brain development is essential to decoding the long-term impact of widespread, poorly defined, and frequently occurring insults such as inflammation. Using the laboratory rat Rattus norvegicus, we have discovered a narrowly constrained critical period
Jay W Chaplin et al.
The Journal of experimental medicine, 210(10), 2135-2146 (2013-09-11)
Antigen (Ag) targeting is an efficient way to induce immune responses. Ag is usually coupled to an antibody (Ab) specific for a receptor expressed on dendritic cells (DCs), and then the Ag-anti-receptor is inoculated with an adjuvant. Here we report
Agnieszka Zdybicka-Barabas et al.
Amino acids, 51(2), 175-191 (2018-09-01)
Cecropins constitute an important family of insect antimicrobial peptides involved in humoral innate immune response. In comparison with the highly basic cecropins A and B, cecropins D are less cationic and more hydrophobic. Interestingly, cecropins D were described only in
Long-Yun Zhou et al.
Journal of neurochemistry, 155(2), 154-176 (2020-03-28)
Cervical spondylotic myelopathy (CSM) is a common cause of disability with few treatments. Aberrant mitochondrial dynamics play a crucial role in the pathogenesis of various neurodegenerative diseases. Thus, regulation of mitochondrial dynamics may offer therapeutic benefit for the treatment of
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