推荐产品
形式
lyophilized powder
比活
≥20 units/mg solid
储存温度
−20°C
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一般描述
Lactate oxidase is a globular flavoprotein and utilizes flavin mononucleotide (FMN) as the cofactor. The enzyme contains a major tyrosine in the substrate-binding pocket and a flavin adenine dinucleotide (FAD) active site. Lactate oxidase has several bacterial sources.
Formerly E.C. number 1.1.3.2
应用
Lactate Oxidase from Pediococcus sp. has been:
- used to functionalize the detection reservoirs for lactate analysis
- used in sequential enzymatic reactions to measure lactate production in the supernatants of microglial cultures
- immobilized onto a carbon electrode to generate a self-powered lactate sensor
生化/生理作用
Lactate oxidase is used in immobilization or enzyme-based labels for designing biochemical analytical devices such as immunosensors. This enzyme catalyzes the oxidation of lactate to produce pyruvate and H2O2. Lactate oxidase is useful in the first-generation biosensor approach for the electrochemical detection of lactate.
单位定义
One unit will oxidize 1.0 μmole of L-lactate to pyruvate and H2O2 per min at pH 6.5 at 37 °C.
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
新产品
Thomas Stoisser et al.
Scientific reports, 6, 27892-27892 (2016-06-16)
L-Lactate oxidase (LOX) belongs to a large family of flavoenzymes that catalyze oxidation of α-hydroxy acids. How in these enzymes the protein structure controls reactivity presents an important but elusive problem. LOX contains a prominent tyrosine in the substrate binding
Ying Chen et al.
Biosensors & bioelectronics, 29(1), 115-118 (2011-08-30)
Detection of analytes in complex biological samples, such as milk and blood, normally requires sample pretreatment. These pretreatment regimes reduce assay throughput and increase testing costs. Technologies that make it possible to eliminate sample pretreatment are of great industrial interest.
Jacob M Goran et al.
Analytical chemistry, 83(21), 8123-8129 (2011-09-29)
Nitrogen-doped carbon nanotubes (N-CNTs) provide a simple, robust, and unique platform for biosensing. Their catalytic activity toward the oxygen reduction reaction (ORR) and subsequent hydrogen peroxide (H(2)O(2)) disproportionation creates a sensitive electrochemical response to enzymatically generated H(2)O(2) on the N-CNT
Dong Chung Kim et al.
ACS nano, 4(3), 1580-1586 (2010-02-26)
This paper reports that a bioarchitecture with two different active enzymes can be fabricated conveniently on a prepatterned surface by highly selective surface-templated layer-by-layer (LBL) assembly by coupling a bilayer of avidin/biotin-lactate oxidase (biotin-LOD) with a bilayer of avidin/biotin-horseradish peroxidase
Lyubov I Kazakova et al.
Analytical and bioanalytical chemistry, 405(5), 1559-1568 (2012-09-13)
The concept of enzyme-assisted substrate sensing based on use of fluorescent markers to detect the products of enzymatic reaction has been investigated by fabrication of micron-scale polyelectrolyte capsules containing enzymes and dyes in one entity. Microcapsules approximately 5 μm in
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