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表单
lyophilized powder
比活
≥20 units/mg solid
储存温度
−20°C
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一般描述
Formerly E.C. number 1.1.3.2
Lactate oxidase is a globular flavoprotein and utilizes flavin mononucleotide (FMN) as the cofactor. The enzyme contains a major tyrosine in the substrate-binding pocket and a flavin adenine dinucleotide (FAD) active site. Lactate oxidase has several bacterial sources.
应用
Lactate Oxidase from Pediococcus sp. has been:
- used to functionalize the detection reservoirs for lactate analysis
- used in sequential enzymatic reactions to measure lactate production in the supernatants of microglial cultures
- immobilized onto a carbon electrode to generate a self-powered lactate sensor
生化/生理作用
Lactate oxidase is used in immobilization or enzyme-based labels for designing biochemical analytical devices such as immunosensors. This enzyme catalyzes the oxidation of lactate to produce pyruvate and H2O2. Lactate oxidase is useful in the first-generation biosensor approach for the electrochemical detection of lactate.
单位定义
One unit will oxidize 1.0 μmole of L-lactate to pyruvate and H2O2 per min at pH 6.5 at 37 °C.
警示用语：
Danger
危险声明
预防措施声明
危险分类
Resp. Sens. 1
储存分类代码
13 - Non Combustible Solids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
法规信息
新产品
Kavita Rathee et al.
Biochemistry and biophysics reports, 5, 35-54 (2015-11-11)
Lactate detection plays a significant role in healthcare, food industries and is specially necessitated in conditions like hemorrhage, respiratory failure, hepatic disease, sepsis and tissue hypoxia. Conventional methods for lactate determination are not accurate and fast so this accelerated the
Lyubov I Kazakova et al.
Analytical and bioanalytical chemistry, 405(5), 1559-1568 (2012-09-13)
The concept of enzyme-assisted substrate sensing based on use of fluorescent markers to detect the products of enzymatic reaction has been investigated by fabrication of micron-scale polyelectrolyte capsules containing enzymes and dyes in one entity. Microcapsules approximately 5 μm in
Marcelo Ricardo Romero et al.
Analytical chemistry, 82(13), 5568-5572 (2010-06-04)
An amperometric sensor for lactate quantification is presented. The developed biosensor requires only 0.2 U of lactate oxidase, which is immobilized in a mucin/albumin hydrogel matrix. By protecting the platinum surface with a Nafion membrane, typical interference related to negatively
Xiang Shen et al.
Organic letters, 14(7), 1744-1747 (2012-03-27)
By making use of the aggregation-induced emission feature of silole 1 and the cascade reactions among l-lactic acid (LA), lactate oxidase (LOD), and dodecanoic hydrazine (DH), a new fluorometric "turn-on" method is developed for the detection of LA.
Parvin Samadi Pakchin et al.
Biosensors & bioelectronics, 122, 68-74 (2018-09-23)
In this work, a novel simple and sensitive electrochemical immunosensor was developed based on lactate oxidase as a single electrochemical probe for the detection of carcinoma antigen 125 (CA125). Chitosan-gold nanoparticle/multiwall carbon nanotube/graphene oxide (CS-AuNP/MWCNT/GO) was used as the electrode
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