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类型
for DNA synthesis
for DNA synthesis
产品线
Proligo Reagents
储存条件
dry at room temperature
浓度
29-32 g/L (DCI content, titration)
杂质
≤30 ppm water content (Karl Fischer)
≤30 ppm water content (Karl Fischer)
颜色
clear
clear
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制备说明
二氰基咪唑/乙腈·3/100 (w/v)
其他说明
Activator solution, containing 4,5-dicyanoimidazole (DCI) in acetonitrile, are mixed with solutions of phosphoramidites during the extension step.
The activator reacts with the amidite group to form a highly reactive intermediate. The intermediate then forms an internucleotide bond with the detritylated 5′hydroxyl group of the growing oligonucleotide chain.
The activator reacts with the amidite group to form a highly reactive intermediate. The intermediate then forms an internucleotide bond with the detritylated 5′hydroxyl group of the growing oligonucleotide chain.
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