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Merck
CN

L0302511

Sigma-Aldrich

ETT 激活剂

别名:

5-乙硫基四氮唑 溶液, 5-(乙硫基)-1 H-四唑, 5-(乙硫基)四唑溶液

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About This Item

MDL编号:
UNSPSC代码:
12352202
NACRES:
NA.21

类型

for DNA synthesis

质量水平

产品线

Proligo Reagents

包装

pkg of 4 × 2.5 L L0302511-04

浓度

39-43 g/L (titration)

杂质

≤30 ppm water content (Karl Fischer)

颜色

clear

SMILES字符串

CCSc1nnn[nH]1

InChI

1S/C3H6N4S/c1-2-8-3-4-6-7-5-3/h2H2,1H3,(H,4,5,6,7)

InChI key

GONFBOIJNUKKST-UHFFFAOYSA-N

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一般描述

ETT activator is an activator solution containing 5-(Ethylthio)-1H-tetrazole – a powerful activator – in acetonitrile. It is mixed with phosphoramidite during the extension step in which it reacts with the amidite group to form a highly reactive intermediate. The intermediate then forms an internucleotide bond with the detritylated 5′hydroxyl group of the growing oligonucleotide chain.

应用

ETT activator has been used in the fast and low-cost synthesis of photolithographic DNA microarrays.

警示用语:

Danger

危险分类

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Irrit. 2

WGK

WGK 3

闪点(°F)

35.6 °F

闪点(°C)

2 °C

法规信息

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Matej Sack et al.
Journal of nanobiotechnology, 14, 14-14 (2016-03-05)
DNA microarrays are a core element of modern genomics research and medical diagnostics, allowing the simple and simultaneous determination of the relative abundances of hundreds of thousands to millions of genomic DNA or RNA sequences in a sample. Photolithographic in

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