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等级
for molecular biology
方案
>99% (SDS-PAGE)
表单
buffered aqueous solution
比活
83,333 U/mg
浓度
100,000 U/mL
运输
dry ice
储存温度
−20°C
一般描述
φ29 DNA Polymerase responsible for the replication of the Bacillus Subtilis phage φ29 (1). The enzyme is a highly processive DNA polymerase (up to 70,000 base insertions per binding event) with a powerful strand displacement activity (2) and a 3′ → 5′ proofreading exonuclease function.
应用
Suitable for Whole Genome Amplification.
特点和优势
- Ultra-purification process for ultimate enzyme performance
- Highest quality specifications for ultimate product consistency
- Undetectable DNA and nuclease contamination
组分
Supplied with:KEM0054B (10X φ29 DNA Polymerase Reaction Buffer)
单位定义
1 unit is defined as the amount of polymerase required to convert 0.5 pmol of dTTP into acid insoluble material in 10 minutes at 30° C.
外形
Supplied in 10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5% TWEEN®-20, 0.5% NP-40, and 50% glycerol at pH 7.4 @ 25° C.
其他说明
Source of protein: A recombinant E. coli strain carrying the φ29 DNA Polymerase gene from bacteriophage φ29.
Unit size: 2,000 U
法律信息
TWEEN is a registered trademark of Croda International PLC
储存分类代码
10 - Combustible liquids
法规信息
新产品
Laure Rittié et al.
Journal of cell communication and signaling, 2(1-2), 25-45 (2008-09-04)
Since molecular cloning has become routine laboratory technique, manufacturers offer countless sources of enzymes to generate and manipulate nucleic acids. Thus, selecting the appropriate enzyme for a specific task may seem difficult to the novice. This review aims at providing
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