grade
Molecular Biology
assay
>99% (SDS-PAGE)
form
buffered aqueous solution
specific activity
83,333 U/mg
concentration
100,000 U/mL
shipped in
dry ice
storage temp.
−20°C
General description
φ29 DNA Polymerase responsible for the replication of the Bacillus Subtilis phage φ29 (1). The enzyme is a highly processive DNA polymerase (up to 70,000 base insertions per binding event) with a powerful strand displacement activity (2) and a 3′ → 5′ proofreading exonuclease function.
Application
Suitable for Whole Genome Amplification.
Features and Benefits
- Ultra-purification process for ultimate enzyme performance
- Highest quality specifications for ultimate product consistency
- Undetectable DNA and nuclease contamination
Physical form
Supplied in 10 mM Tris-HCl, 100 mM KCl, 1 mM DTT, 0.1 mM EDTA, 0.5% TWEEN®-20, 0.5% NP-40, and 50% glycerol at pH 7.4 @ 25° C.
Other Notes
1 unit is defined as the amount of polymerase required to convert 0.5 pmol of dTTP into acid insoluble material in 10 minutes at 30° C.
Source of protein: A recombinant E. coli strain carrying the φ29 DNA Polymerase gene from bacteriophage φ29.
Supplied with:KEM0054B (10X φ29 DNA Polymerase Reaction Buffer)
Unit size: 2,000 U
Legal Information
TWEEN is a registered trademark of Croda International PLC
存储类别
10 - Combustible liquids
法规信息
新产品
此项目有
Laure Rittié et al.
Journal of cell communication and signaling, 2(1-2), 25-45 (2008-09-04)
Since molecular cloning has become routine laboratory technique, manufacturers offer countless sources of enzymes to generate and manipulate nucleic acids. Thus, selecting the appropriate enzyme for a specific task may seem difficult to the novice. This review aims at providing
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持