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表单
liquid
用途
sufficient for 500 reactions
特点
dNTPs included
hotstart
储存条件
protect from light
技术
qPCR: suitable
颜色
light blue
输入
purified RNA
相容性
ABI 7500 Fast
ABI 7500
ABI QuantStudio
ABI ViiA 7
Agilent AriaMx
Douglas Scientific IntelliQube
Qiagen Corbett Rotor-Gene Q
Strategene Mx3000P
Strategene Mx3005P
Strategene Mx4000
检测方法
probe-based
运输
dry ice
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此商品 | 230464 | 371491 | 293121 |
|---|---|---|---|
| Quality Level 200 | Quality Level 200 | Quality Level - | Quality Level 100 |
| mp -104.5 °C | mp −105 °C (lit.) | mp −105 °C (lit.) | mp - |
| density 1.64 g/cm3 at 20 °C | density 1.631 g/mL at 25 °C (lit.) | density 1.631 g/mL at 25 °C (lit.) | density 1.373 g/mL at 25 °C |
| vapor pressure 121 hPa ( 20 °C) | vapor pressure 97 mmHg ( 20 °C) | vapor pressure 97 mmHg ( 20 °C) | vapor pressure 97 mmHg ( 20 °C) |
| potency 324 mg/kg LD50, oral (Rat) | potency - | potency - | potency - |
一般描述
KiCqStart® One-Step Probe RT-qPCR ReadyMix™ is a ready-to-use, highly sensitive master mix for reverse transcription quantitative PCR (RT-qPCR) of RNA templates using hybridization probe detection chemistries such as TaqMan® 5′-hydrolysis probes on real-time PCR systems (refer to instrument compatibility section to select the appropriate reagent for your machine). First-strand cDNA synthesis and PCR amplification are carried out in the same tube without opening between procedures. It is ideal for highly sensitive quantification of RNA viruses or low abundance RNA targets as well as high throughput gene-expression studies. The system has been optimized to deliver maximum RT-PCR efficiency, sensitivity, and specificity in reduced reaction volumes and fast cycle times.
KiCqStart® One-Step Probe RT-qPCR ReadyMix™ contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries. The reverse transcriptase is an engineered M-MLV with reduced RNase H activity and improved activity and stability at higher temperatures. The use of higher temperatures (50 to 55°C) for the first-strand step of one-step RT-qPCR provides higher specificity for primer annealing and disruption of RNA secondary structure that can interfere with cDNA synthesis.
KiCqStart® One-Step Probe RT-qPCR ReadyMix™ is highly resistant to PCR inhibitors. A key component of the ReadyMix™ is an ultra-pure, highly processive, thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This provides an extremely stringent automatic hot-start that minimizes the potential for primer-dimer and other non-specific PCR artifacts. The light blue color of the inert dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. The dye does not interfere with qPCR performance or affect the stability of the product.
KiCqStart® One-Step Probe RT-qPCR ReadyMix™ contains all required components for RT-qPCR except RNA template and probe. It is compatible with all dual-labeled probe chemistries. The reverse transcriptase is an engineered M-MLV with reduced RNase H activity and improved activity and stability at higher temperatures. The use of higher temperatures (50 to 55°C) for the first-strand step of one-step RT-qPCR provides higher specificity for primer annealing and disruption of RNA secondary structure that can interfere with cDNA synthesis.
KiCqStart® One-Step Probe RT-qPCR ReadyMix™ is highly resistant to PCR inhibitors. A key component of the ReadyMix™ is an ultra-pure, highly processive, thermostable DNA polymerase that is combined with high avidity monoclonal antibodies. This provides an extremely stringent automatic hot-start that minimizes the potential for primer-dimer and other non-specific PCR artifacts. The light blue color of the inert dye simplifies reaction assembly in white, or clear, plates and helps to minimize pipetting or mixing errors. The dye does not interfere with qPCR performance or affect the stability of the product.
应用
Different real-time PCR systems employ different strategies for normalization of fluorescent signals and correction of well-to-well optical variations. It is critical to match the appropriate qPCR reagent to your specific instrument.KiCqStart® One-Step Probe RT-qPCR ReadyMix™ does not contain an internal reference dye. Compatible instruments include:
- Applied Biosystems® 7500
- Applied Biosystems 7500 Fast
- Applied Biosystems ViiA 7
- Applied Biosystems QuantStudio
- Agilent® AriaMx
- Douglas Scientific IntelliQube
- QIAGEN® Rotor-Gene® Q
- Stratagene Mx3000P®
- Stratagene Mx3005P®
- Stratagene Mx4000™
特点和优势
- 仅需33分钟即可获得检测结果
- 高效和高灵敏度的实时PCR结果
- 极少/无需优化
组分
2X reaction buffer containing dATP, dCTP, dGTP, dTTP, magnesium chloride, reverse transcriptase, RNase inhibitor protein, hot-start DNA polymerase, ROX reference dye, inert blue qPCR dye, and stabilizers
packaging:
500 reactions* = 5 X 1 mL tubes
*number of reactions based on a 20uL volume
packaging:
500 reactions* = 5 X 1 mL tubes
*number of reactions based on a 20uL volume
法律信息
Agilent is a registered trademark of Agilent Technologies, Inc.
Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
KiCqStart is a registered trademark of QIAGEN Beverly Inc.
Mx3000P is a registered trademark of Agilent Technologies, Inc.
Mx3005P is a registered trademark of Agilent Technologies, Inc.
Mx4000 is a trademark of Agilent Technologies, Inc.
Qiagen is a registered trademark of Qiagen Group
ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
ReadyMix is a trademark of Sigma-Aldrich Co. LLC
Rotor-Gene is a registered trademark of Qiagen GmbH
TaqMan is a registered trademark of Roche Molecular Systems, Inc.
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产品编号
说明
价格
储存分类代码
10 - Combustible liquids
WGK
WGK 3
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
常规特殊物品
Tyler J Kirby et al.
Molecular biology of the cell, 27(5), 788-798 (2016-01-15)
Myofibers increase size and DNA content in response to a hypertrophic stimulus, thus providing a physiological model with which to study how these factors affect global transcription. Using 5-ethynyl uridine (EU) to metabolically label nascent RNA, we measured a sevenfold
Tyler J Kirby et al.
Physiological genomics, 48(2), 145-153 (2015-12-17)
Adipose tissue has profound effects on whole-body insulin sensitivity. However, the underlying biological processes are quite complex and likely multifactorial. For instance, the adipose transcriptome is posttranscriptionally modulated by microRNAs, but the relationship between microRNAs and insulin sensitivity in humans
R Grace Walton et al.
Physiological reports, 3(6), doi:10-doi:10 (2015-06-04)
Reduced vessel density in adipose tissue and skeletal muscle is associated with obesity and may result in decreased perfusion, decreased oxygen consumption, and insulin resistance. In the presence of VEGFA, Angiopoietin-2 (Angpt2) and Angiopoietin-1 (Angpt1) are central determinants of angiogenesis
商品
RT-qPCR products combine the effective of Reverse Transcriptase with hot-start taq-directed antibody in convenient ReadyMixes for probe-based or SYBR® Green based applications.
PCR assay guide navigates you through primer validation and other assay optimization factors to ensure high sensitivity and specificity for optimum DNA/ RNA quantification.
PCR测定指南可指导您全程了解引物验证和其他测定优化因素，确保实现理想的DNA / RNA定量高灵敏度和特异性。
分析基因表达的方法之一是测量基因的mRNA浓度。此类分析面临着若干挑战，例如不同转录本之间的半衰期不同、转录时间模式以及mRNA和蛋白质之间缺乏相关性。
实验方案
KiCqStart One-Step Probe RT-qPCR ReadyMix streamlines RNA template RT-qPCR with high sensitivity.
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