推荐产品
生物来源
rat
偶联物
unconjugated
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
C3H7D2, monoclonal
表单
buffered aqueous solution
分子量
antigen ~100 kDa
种属反应性
mouse
浓度
~2 mg/mL
技术
immunocytochemistry: suitable using methanol-acetone fixation
microarray: suitable
western blot: 0.5-1 μg/mL using a whole extract of transfected 293T (human embryonal kidney) cells expressing mouse KSR
同位素/亚型
IgG1
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
mouse ... Ksr1(16706)
一般描述
Kinase Suppressor of Ras (KSR) is a positive effector of Ras signalling pathway. Initially discovered in Drosophila and C. elegans, the KSR family of proteins is structurally similar but distinct from the Raf Family. In response to Ras activation, KSR translocates from the cytoplasm to the plasma membrane and may act as a scaffolding protein to assemble a MAPK signalling complex. KSR interacts with Raf-1, MEK1/2 and ERK1/2 and this interaction is required for a signalling cascade downstream of Ras. Thus KSR plays an important role in survival, proliferation, differentiation, growth and transformation of cells. KSR mediates Ras-dependent signals that are required for neuronal differentiation and functioning of central nervous system
Monoclonal Anti-KSR specifically reacts with mouse KSR (~100 kDa).
Monoclonal Anti-KSR specifically reacts with mouse KSR (~100 kDa).
免疫原
recombinant KSR fragment (amino acids 118-248) from mouse.
应用
Anti-KSR is suitable for immunoblotting at a working concentration of 0.5-1.0 μg/mL using human 293T cells expressing mouse KSR. The antibody may be used for immunocytochemistry and protein microarray applications.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
制备说明
Purified from culture supernatant of hybridoma cells grown in a bioreactor.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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法规信息
新产品
D K Morrison
Journal of cell science, 114(Pt 9), 1609-1612 (2001-04-20)
Kinase Suppressor of Ras (KSR) is an intriguing component of the Ras pathway that was first identified by genetic studies performed in Drosophila melanogaster and Caenorhabditis elegans. In both organisms, inactivating mutations in KSR suppress the phenotypic effects induced by
K Kornfeld et al.
Cell, 83(6), 903-913 (1995-12-15)
By screening for mutations that suppress the vulval defects caused by a constitutively active let-60 ras gene, we identified six loss-of-function alleles of ksr-1, a novel C. elegans gene. Our genetic analysis showed ksr-1 positively mediates Ras signaling and functions
N R Michaud et al.
Proceedings of the National Academy of Sciences of the United States of America, 94(24), 12792-12796 (1997-12-16)
Kinase suppressor of Ras (KSR) is an evolutionarily conserved component of Ras-dependent signaling pathways. Here, we find that murine KSR (mKSR1) translocates from the cytoplasm to the plasma membrane in the presence of activated Ras. At the membrane, mKSR1 modulates
J Müller et al.
Molecular and cellular biology, 20(15), 5529-5539 (2000-07-13)
Kinase suppressor of Ras (KSR) is an evolutionarily conserved component of Ras-dependent signaling pathways. Here, we report the identification of B-KSR1, a novel splice variant of murine KSR1 that is highly expressed in brain-derived tissues. B-KSR1 protein is detectable in
S Stewart et al.
Molecular and cellular biology, 19(8), 5523-5534 (1999-07-20)
Genetic screens for modifiers of activated Ras phenotypes have identified a novel protein, kinase suppressor of Ras (KSR), which shares significant sequence homology with Raf family protein kinases. Studies using Drosophila melanogaster and Caenorhabditis elegans predict that KSR positively regulates
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