生物来源
rabbit
偶联物
unconjugated
抗体形式
affinity isolated antibody
抗体产品类型
primary antibodies
克隆
polyclonal
表单
buffered aqueous solution
分子量
antigen ~90 kDa
种属反应性
human, rat, mouse
增强验证
recombinant expression
Learn more about Antibody Enhanced Validation
浓度
~1 mg/mL
技术
immunoprecipitation (IP): 5-10 μg using mouse brain extract (S1 fraction).
western blot: 0.5-1 μg/mL using HEK-293T cells expressing human PIP5K1C
western blot: 1-2 μg/mL using rat brain (S1 fraction)
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... PIP5K1C(23396)
mouse ... Pip5k1c(18717)
rat ... Pip5k1c(314641)
一般描述
Phosphatidylinositol-4-phosphate-5-kinase, type 1γ (PIP5K1C) belongs to the type I phosphatidylinositol-4-phosphate-5-kinases. This protein is expressed at high levels in the brain and is concentrated at synapses. The PIP5K1C gene is located on the human chromosome at 19p13.3.
特异性
Anti-PIP5K1C (N-terminal) specifically recognizes human, rat, and mouse PIP5K1C.
应用
Anti-PIP5K1C (N-terminal) antibody produced in rabbit may be used in immunoblotting and immunoprecipitation.
生化/生理作用
Phosphatidylinositol-4-phosphate-5-kinase, type 1γ (PIP5K1C) is involved in phosphorylating phosphatidylinositol-4-phosphate (PtdInsP) to generate phosphatidylinositol-4,5-bisphosphate (PtdIns(4,5)P2). PIP5K1C has been shown to bind to the 4.1/ezrin/radixin/moesin (FERM) domain of talin, which contains also the binding sites of β-integrin, actin, and PtdIns(4,5)P2. PIP5K1C is recruited to the membrane by talin at neuronal synapses may be required to generate the PtdIns(4,5)P2 pool involved in clathrin coating and actin dynamics during vesicle recycling. Mutations in the PIP5K1C gene is associated with lethal congenital contracture syndrome type 3 (LCCS3), which is an intense form of arthrogryposis.
外形
0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。
储存及稳定性
Store at –20 °C. For continuous use, the product may be stored at 2–8 °C for up to one month. For extended storage, freeze in working aliquots at –20 °C. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilutions should be discarded if not used within 12 hours.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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法规信息
新产品
Alexander Wallroth et al.
The Journal of biological chemistry, 293(5), 1526-1535 (2017-12-29)
Phosphoinositides (PIs) are phospholipids that perform crucial cell functions, ranging from cell migration and signaling to membrane trafficking, by serving as signposts of compartmental membrane identity. Although phosphatidylinositol 4,5-bisphosphate, 3-phosphate, and 3,5-bisphosphate are commonly considered as hallmarks of the plasma
Gilbert Di Paolo et al.
Nature, 420(6911), 85-89 (2002-11-08)
Membrane phosphoinositides control a variety of cellular processes through the recruitment and/or regulation of cytosolic proteins. One mechanism ensuring spatial specificity in phosphoinositide signalling is the targeting of enzymes that mediate their metabolism to specific subcellular sites. Phosphatidylinositol phosphate kinase
Guiqing Lu et al.
American journal of cancer research, 10(10), 3382-3394 (2020-11-10)
Endosomes regulate cell polarity, adhesion, signaling, immunity, and tumor progression, which may influence cancer outcomes. Here we evaluated associations between 36,068 genetic variants of 228 endosome-related pathway genes and cutaneous melanoma disease-specific survival (CMSS) using genotyping data from two previously
Ginat Narkis et al.
American journal of human genetics, 81(3), 530-539 (2007-08-19)
Lethal congenital contractural syndrome (LCCS) is a severe form of arthrogryposis. To date, two autosomal recessive forms of the disease (LCCS and LCCS2) have been described and mapped to chromosomes 9q34 and 12q13, respectively. We now describe a third LCCS
Xiaojie Yu et al.
Proceedings of the National Academy of Sciences of the United States of America, 119(45), e2210618119-e2210618119 (2022-11-03)
Alterations of the tumor suppressor TP53, one of the most common events in cancer, alone are insufficient for tumor development but serve as drivers of transformation. We sought to identify cooperating events through genomic analyses of a somatic Trp53R245W mouse
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