推荐产品
一般描述
αα-酮戊二酸脱氢酶(α-KGDH)是位于线粒体的一种多酶复合体。这种复合酶由多个单位的硫胺素焦磷酸依赖性脱氢酶(E1)、二氢硫辛酰脱氢酶(E3)和二氢硫辛酰转琥珀酰酶(E2)组成。
应用
α猪心α-酮戊二酸脱氢酶已用于:
- 研究过氧亚硝酸盐处理细胞中谷胱甘肽(GSH)的反硝化作用
- 通过Spectramax M5微孔板分光荧光计检测其在心肌线粒体中的活性
- 作为阳性对照,通过Spectramax GEMINI EM荧光微孔板读板机评估其在小鼠神经元中的活性
生化/生理作用
αα-酮戊二酸脱氢酶(α-KGDH)是生物能量学过程的关键酶,是Krebs循环或三羧酸(TCA)循环代谢流的调控物。它通过还原型烟酰胺腺嘌呤二核苷酸(NADH)的释放,催化α-酮戊二酸氧化脱羧为琥珀酰-CoA。这是TCA循环中的限速反应。该反应为呼吸链提供电子,需要硫胺素焦磷酸作为辅因子。反应速率由NAD(烟酰胺腺嘌呤二核苷酸)还原决定。αα-KGDH的最佳pH值范围为6.6-7.4。该酶会被氧化应激抑制并导致代谢缺乏。但α-KGDH又能产生活性氧(ROS)导致氧化应激。α-KGDH缺陷或浓度不够会导致好几种神经退行性疾病,比如阿尔茨海默病。
质量
可能含有痕量的聚乙二醇。
单位定义
在 30 °C、pH 7.4、饱和水平的辅酶 A 存在的条件下,一单位每分钟可将 1.0 μ 摩尔的 β-NAD 转化成 β-NADH。
外形
以 50% 甘油溶液的形式提供,含有 9mg 每毫升的牛血清白蛋白、30% 蔗糖、1.5 mM EDTA、1.5 mM EGTA、1.5 mM 2-巯基乙醇、0.3% TRITON™ X-100、0.003% 叠氮化钠和 15 mM 磷酸钾,pH 6.8。
法律信息
Triton is a trademark of The Dow Chemical Company or an affiliated company of Dow
危险声明
预防措施声明
危险分类
Aquatic Chronic 3
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
含少量动物源组分生物产品
常规特殊物品
Science (New York, N.Y.), 334(6062), 1551-1553 (2011-12-17)
It is generally accepted that cyanobacteria have an incomplete tricarboxylic acid (TCA) cycle because they lack 2-oxoglutarate dehydrogenase and thus cannot convert 2-oxoglutarate to succinyl-coenzyme A (CoA). Genes encoding a novel 2-oxoglutarate decarboxylase and succinic semialdehyde dehydrogenase were identified in
The Journal of biological chemistry, 289(12), 8312-8325 (2014-02-12)
Several flavin-dependent enzymes of the mitochondrial matrix utilize NAD(+) or NADH at about the same operating redox potential as the NADH/NAD(+) pool and comprise the NADH/NAD(+) isopotential enzyme group. Complex I (specifically the flavin, site IF) is often regarded as
?-ketoglutarate dehydrogenase from pig heart
Test, 13, 52-55 (1969)
Journal of neurochemistry, 134(1), 86-96 (2015-03-17)
Reversible post-translation modifications of proteins are common in all cells and appear to regulate many processes. Nevertheless, the enzyme(s) responsible for the alterations and the significance of the modification are largely unknown. Succinylation of proteins occurs and causes large changes
Trends in plant science, 17(9), 503-509 (2012-06-05)
As a fundamental energy-conserving process common to all living organisms, respiration is responsible for the oxidation of respiratory substrates to drive ATP synthesis. Accordingly, it has long been accepted that a complete tricarboxylic acid (TCA) cycle is necessary for respiratory
我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系技术服务部门