Phagocytosis Assay Kit

Phagocytosis is a cellular process for ingesting and eliminating microorganisms, foreign substances, and apoptotic cells. It is a specific form of endocytosis initiated by recognition and binding of foreign particles by cell surface receptors, followed by their engulfment, and formation of phagosomes. Maturing phagosomes transform to phagolysosomes which destroy the pathogen through enzymes and toxic peroxides. E. coli and other bacterial strains are often used as pathogens in phagocytosis assays.

The Phagocytosis Assay Kit (Green E. coli) uses heat-killed, fluorescently pre-labeled E. coli particles as a tool to detect and quantify in vitro phagocytosis by fluorescent microscope, spectrophotometer (Ex/Em = 490/520 nm) or flow cytometry.

This kit is suitable for screening activators and/or inhibitors of phagocytosis and Toll-like receptor ligands (TLR) in mammalian cells.