Anti-Importin α1 antibody, Rat monoclonal

Monoclonal Anti-Importin α1 (rat IgG2α isotype) is derived from the hybridoma 1A6 produced by the fusion of mouse myeloma cells (SP2/0), and splenocytes from BN/Crj rats.

The KPNA2 (karyopherin subunit α 2) consists of 529 amino acids and has a molar mass of 58kDa. The protein consists of an N-terminal hydrophilic importin β-binding domain that has an auto-inhibitory function to ensure the translocation of KPNA2 occurs only when it is bound to importin β and a cargo molecule. It also contains a central hydrophobic region made up of 10 armadillo (ARM) repeats, which binds the cargo′s NLS (nuclear localization signal) and CAS, the factor involved in the recycling of KPNA2 to the cytoplasm. The C-terminus has a short acidic region whose function is yet to be determined. The gene is mapped to human chromosome 17q24.2.

Monoclonal Anti-Importin a1 recognizes human, canine, mouse, and chicken Importin a1 (approx. 60kDa)

recombinant mouse importin α1.

Monoclonal Anti-Importin α1 antibody produced in rat has been used in:

• immunoblotting
• immunofluorescence
• immunocytochemistry

The gene KPNA2 (karyopherin subunit α 2), also referred to as importin α1, encodes a member of the importin α family. It participates in the normal nuclear localization of the MRN (MRE11-RAD50-NBS1) complex. It also facilitates proper formation of the nuclear foci of the complex. KPNA2 is the sole member of its family to interact with NBS1 (Nijmegen breakage syndrome 1), a component of the MRN complex. It also functions in the tumor suppression functions associated with NBS1 complex. Inhibition of the interaction between KPNA2 and NBS1 can lead to decreased radiation-induced nuclear focus accumulation, DSB (double strand break) repair, and cell cycle checkpoint signaling of NBS1. KPNA2 is found to regulate epidermal proliferation and differentiation and participates in the activation of cellular signaling in blood lymphocytes. It has been linked to Russell-Silver syndrome. Overexpression of this protein has been associated with several types of cancer and it may serve as a potential biomarker.

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

For continuous use, store at 2-8 °C for up to one month. For prolonged storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. Storage in frost-free freezers is also not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

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