推荐产品
等级
for molecular biology
质量水平
产品线
BioReagent
方案
≥98.5%
表单
liquid
技术
RNA extraction: suitable
pH值(酸碱度)
5.6 (20 °C, 25 g/L)
密度
0.809 g/mL at 20 °C (lit.)
适用性
suitable for nucleic acid purification
储存温度
room temp
SMILES字符串
CC(C)CCO
InChI
1S/C5H12O/c1-5(2)3-4-6/h5-6H,3-4H2,1-2H3
InChI key
PHTQWCKDNZKARW-UHFFFAOYSA-N
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一般描述
3-甲基丁醇(异戊醇)是一种透明、无色的醇,常用作有机溶剂。也可用于酚-氯仿核酸提取和纯化方案中,在此过程中抑制 RNase 活性,减少起泡。异戊醇也可用于从 CsCl 梯度超速离心纯化的 DNA 中去除溴化乙锭。
应用
3-甲基丁醇已被用作苯酚/氯仿/异戊醇溶液的成分,用于从血液以及吸收基质法医样品和生物膜中提取DNA。
3-甲基丁醇(异戊醇)与苯酚和氯仿联合用于提取 RNA。它被用于使用光敏腺苷分子合成低聚核苷酸-RNA。
警示用语:
Danger
危险分类
Acute Tox. 4 Inhalation - Eye Dam. 1 - Flam. Liq. 3 - Skin Irrit. 2 - STOT SE 3
靶器官
Respiratory system
储存分类代码
3 - Flammable liquids
WGK
WGK 1
闪点(°F)
110.3 °F - closed cup
闪点(°C)
43.5 °C - closed cup
个人防护装备
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
法规信息
危险化学品
DNA extraction from blood and forensic samples
Guidelines for Molecular Analysis in Archive Tissues, 45-54 (2011)
Section 1 Isolation of nucleic acids
Biofouling Methods, 88-88 (2014)
Nature protocols, 2(5), 1052-1058 (2007-06-05)
This protocol describes a general method for the preparation of RNAs in which the reactivity or hydrogen-bonding properties of the molecule are modified in a photoreversible fashion by use of a caging strategy. A single caged adenosine, modified at the
Molecular systems biology, 7, 487-487 (2011-05-11)
Many compounds being considered as candidates for advanced biofuels are toxic to microorganisms. This introduces an undesirable trade-off when engineering metabolic pathways for biofuel production because the engineered microbes must balance production against survival. Cellular export systems, such as efflux
Food microbiology, 27(7), 945-954 (2010-08-07)
Differences in the production of bacterial metabolites with potential impact on fermented sausage flavour were found in meat simulation medium when comparing different strains of Staphylococcus xylosus and Staphylococcus carnosus as starter cultures. Overall, higher levels of 3-methyl-1-butanol and acetoin
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