I9018
抗-整合素单克隆抗体 小鼠抗
clone SY5, ascites fluid
别名:
抗外皮蛋白
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关于此项目
Conjugate:
unconjugated
Clone:
SY5, monoclonal
Application:
immunocytochemistry
immunohistochemistry (formalin-fixed, paraffin-embedded sections)
immunohistochemistry (frozen sections)
immunoprecipitation (IP)
indirect ELISA
indirect immunofluorescence
western blot
immunohistochemistry (formalin-fixed, paraffin-embedded sections)
immunohistochemistry (frozen sections)
immunoprecipitation (IP)
indirect ELISA
indirect immunofluorescence
western blot
Species reactivity:
canine, gorilla, owl monkey, pig, human
Citations:
83
Technique(s):
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
indirect immunofluorescence: 1:100 using formalin-fixed, paraffin-embedded human skin sections
western blot: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
indirect immunofluorescence: 1:100 using formalin-fixed, paraffin-embedded human skin sections
western blot: suitable
Uniprot accession no.:
产品名称
抗-整合素单克隆抗体 小鼠抗, clone SY5, ascites fluid
biological source
mouse
conjugate
unconjugated
antibody form
ascites fluid
antibody product type
primary antibodies
clone
SY5, monoclonal
contains
15 mM sodium azide
species reactivity
canine, gorilla, owl monkey, pig, human
technique(s)
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
indirect immunofluorescence: 1:100 using formalin-fixed, paraffin-embedded human skin sections
western blot: suitable
isotype
IgG1
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
target post-translational modification
unmodified
Quality Level
Gene Information
human ... IVL(3713)
Application
单克隆抗外皮蛋白抗体适用于使用角质形成细胞进行免疫荧光（1:100稀释），或使用甲醇固定的小鼠胚胎成纤维细胞-角质形成细胞共培养物进行间接免疫荧光。该抗体还可用于蛋白质印迹 (1:1000) 和免疫组化分析。此外，该产品还可用于间接ELISA、免疫印迹、免疫沉淀和免疫细胞化学。
Biochem/physiol Actions
该抗体可用于各种技术检测灵长类和非灵长类哺乳动物的外皮蛋白。 在培养的人角质形成细胞中，在变性还原条件下，该抗体通过免疫印迹染色出120 kDa条带。但在其他细胞中，该抗体染色出其他大小的外皮蛋白：在人乳腺癌细胞系 MCF-7 中为 170 kDa，在大猩猩和猫头鹰猴为双条带约为 115 和 150 kDa，在狗体内为 66 kDa，在猪体内的双条带约为 105 kDa。该抗体不与小鼠表皮反应，因此可用于研究裸鼠体内的人类异种移植物。该抗体对人皮肤的上棘层和颗粒层以及分层克隆中的基底上终末分化角质形成细胞的细胞质进行染色。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
General description
外皮蛋白是一种可溶性前体蛋白，调节角质形成细胞的终末分化。该蛋白质最初存在于角质形成细胞的胞质成分中，随后与膜蛋白结合。外皮蛋白在鳞状细胞癌中表达，其表达被HPV 16癌蛋白下调。单克隆抗外皮蛋白对灵长类和非灵长类哺乳动物的外皮蛋白具有特异性。在培养的人角质形成细胞中，在变性还原条件下，该抗体通过免疫印迹染色出120 kDa条带。然而，在其他细胞中，抗体与不同大小的外皮蛋白结合：在人乳腺癌细胞系 MCF-7 中为 170 kDa，在大猩猩和猫头鹰猴为双条带约为 115 和 150 kDa，在狗体内为 66 kDa，在猪体内的双条带约为 105 kDa。该抗体不与小鼠表皮结合；因此该抗体可用于研究裸鼠体内的人类异种移植物。该抗体对人皮肤的上棘层和颗粒层以及分层克隆中的基底上终末分化角质形成细胞的细胞质进行染色。
Immunogen
人囊包蛋白。
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存储类别
10 - Combustible liquids
wgk
nwg
flash_point_f
Not applicable
flash_point_c
Not applicable
M N Ruesch et al.
Journal of virology, 72(6), 5016-5024 (1998-05-30)
The life cycle of the papillomaviruses is closely linked to host cell differentiation, as demonstrated by the fact that amplification of viral DNA and transcription of late genes occur only in the suprabasal cells of a differentiated epithelium. Previous studies
Jun Dai et al.
PloS one, 8(7), e70392-e70392 (2013-08-08)
RORα is a retinoid-related orphan nuclear receptor that regulates inflammation, lipid metabolism, and cellular differentiation of several non-epithelial tissues. In spite of its high expression in skin epithelium, its functions in this tissue remain unclear. Using gain- and loss-of-function approaches
N Fabre-Jonca et al.
The Journal of investigative dermatology, 112(2), 216-225 (1999-02-16)
Microtubules are involved in the positioning and movement of organelles and vesicles and therefore play fundamental roles in cell polarization and differentiation. Their organization and properties are cell-type specific and are controlled by microtubule-associated proteins (MAP). E-MAP-115 (epithelial microtubule-associated protein
Z Suo et al.
Histopathology, 23(1), 45-54 (1993-07-01)
The expression of cytokeratins (CK) 1, 4, 5/6, 8, 13, 18, 19 and 20 and involucrin in 42 cases of squamous cell carcinomas from various locations was examined. The tumours expressed CK5/6 in 55%, CK8 in 76%, CK13 in 43%
T Klymenko et al.
Journal of virology (2017-10-13)
The human papillomavirus (HPV) replication cycle is tightly linked to epithelial cell differentiation. To examine HPV-associated changes in the keratinocyte transcriptome, RNAs isolated from undifferentiated and differentiated cell populations of normal, spontaneously immortalised, keratinocytes (NIKS), and NIKS stably transfected with
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