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一般描述
Interleukin-4 (IL-4) is a T cell derived chemokine that stimulates the Th2 mediated immune response and proliferation of B cells. The effects of IL-4 are mediated by two types of receptors, type I receptor consisting of IL-4Rα and common γ chain and type II receptor composed of IL-4Rα and IL-13Rα1. The signaling stimulated by IL-4 leads to activation of JAK/STAT6 and IRS-mediated PI3K/Akt pathway. Through these pathways, IL-4 is responsible for endocytic activity of macrophages, chemotaxis of leukocytes in response to inflammation, angiogenesis and regulation of nitric oxide metabolism in macrophages. Anti-tumor effects of IL-4 have been reported in cancers of breast, liver and renal cells. Anti-Interleukin-4 specifically recognizes human interleukin-4. The antibody shows no cross reactivity with rmIL-4 and rrIL-4.
特异性
The antibody will neutralize the biological activity of recombinant human IL-4, but not recombinant mouse IL-4.
免疫原
recombinant human IL-4.
应用
Anti-Interleukin-4 antibody may be used for immunoblotting at a working concentration of 0.1-0.2 μg/ml. For ELISA, a working concentration of 0.5-1.0 μg/ml is recommended. The antibody may be used for immunohistochemistry of cultured cells or tissue sections at a working antibody concentration of 0.5-5 μg/ml. The antibody is suitable for neutralization assay; ND50 is 0.02-0.1 μg/ml. Anti-IL-4 antibody was used for immunohistochemistry and ELISA using cervicovaginal tissue sections and cervicovaginal extracts of Belgium white rabbits.
Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)
Immunohistochemistry (1 paper)
外形
Lyophilized from 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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相关产品
产品编号
说明
价格
储存分类代码
11 - Combustible Solids
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
个人防护装备
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
Toll-like receptors and cytokines as surrogate biomarkers for evaluating vaginal immune response following microbicide administration
Gupta SM et al
Mediators of Inflammation, doi: 10-doi: 10 (2008)
Investigational therapeutics targeting the IL-4/IL-13/STAT-6 pathway for the treatment of asthma
Oh CK et al
European Respiratory Review, 19, 11546-11554 (2010)
M Stein et al.
The Journal of experimental medicine, 176(1), 287-292 (1992-07-01)
Expression of the macrophage mannose receptor is inhibited by interferon gamma (IFN-gamma), a T helper type 1 (Th-1)-derived lymphokine. Interleukin 4 (IL-4), a Th-2 lymphocyte product, upregulates major histocompatibility class II antigen expression but inhibits inflammatory cytokine production by macrophages.
Alberto Mantovani et al.
Trends in immunology, 25(12), 677-686 (2004-11-09)
Plasticity and functional polarization are hallmarks of the mononuclear phagocyte system. Here we review emerging key properties of different forms of macrophage activation and polarization (M1, M2a, M2b, M2c), which represent extremes of a continuum. In particular, recent evidence suggests
E M Coccia et al.
International immunology, 12(7), 977-985 (2000-07-06)
NO is a labile radical involved in several immunological, antimicrobial and inflammatory processes. In macrophages, NO formation is catalyzed by the cytokine-inducible enzyme inducible NO synthase (iNOS). The importance of IFN regulatory factor (IRF)-1 and of the signal transducers and
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