一般描述
IgG抗体亚型是免疫系统中最丰富的血清免疫球蛋白。它由B细胞分泌,存在于血液和细胞外液中,可防止细菌、真菌和病毒引起的感染。母体IgG通过胎盘转移到胎儿,这对于新生儿抵抗感染的免疫防御至关重要。IgG3具有独特的延伸铰链区,具备更大的灵活性。
通过分离,离子交换和亲和层析程序从人血浆中纯化人骨髓瘤IgG3。
通过分离,离子交换和亲和层析程序从人血浆中纯化人骨髓瘤IgG3。
应用
来自人骨髓瘤血浆的IgG3κ已被用于测定商用治疗性IgG1抗体和人骨髓瘤IgG1、IgG2、IgG3和IgG4抗体中的三硫化物水平。
纯化的IgG3κ可在多种免疫测定中用作免疫球蛋白校准物、参考抗原、封闭剂或包被蛋白,包括ELISA、斑点印迹免疫结合、Western免疫印迹、免疫扩散、免疫电泳、血细胞凝集和细胞结合测定。人骨髓瘤IgG3用于CHO细胞的流式细胞术。
外形
溶于 20 mM tris 缓冲盐水 (pH 8.0) 中。
分析说明
通过免疫电泳、间接ELISA和SDS-PAGE可确定纯度和同一性。
免责声明
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
WGK
WGK 1
闪点(°F)
Not applicable
闪点(°C)
Not applicable
法规信息
常规特殊物品
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Clearance of pathogens or tumor cells by antibodies traditionally requires both Fab and Fc domains of IgG. Here, we show the Fc domain of IgG alone mediates recognition and clearance of herpes simplex virus (HSV1)-infected cells. The human natural killer
Nature immunology, 19(9), 1001-1012 (2018-08-15)
Immunoglobulin G3 (IgG3) has an uncertain role in the response to infection with and vaccination against human immunodeficiency virus (HIV). Here we describe a regulatory role for IgG3 in dampening the immune system-activating effects of chronic HIV viremia on B
Journal of immunology (Baltimore, Md. : 1950), 183(12), 8216-8224 (2009-12-17)
CD32A, the major phagocytic FcgammaR in humans, exhibits a polymorphism in the ligand binding domain. Individuals homozygous for the R allelic form of CD32A (CD32A(R) allele) are more susceptible to bacterial infections and autoimmune diseases as compared with H allelic
Nature immunology, 8(7), 762-771 (2007-06-15)
Because functional analysis of Fc receptors (FcRs) relies heavily on mouse models, the identification of another Fcgamma receptor is particularly noteworthy. We demonstrate that FcgammaRIV, identified here as the mouse ortholog of primate FcgammaRIII, required association of the FcR gamma-chain
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