biological source
sheep
Quality Level
conjugate
unconjugated
antibody form
purified immunoglobulin
grade
reagent grade
assay
≥95% (SDS-PAGE)
form
essentially salt-free, lyophilized powder
isotype
IgG
solubility
saline: 1 mg/mL at -20 °C (Repeated freezing and thawing is not recommended.)
storage temp.
2-8°C
General description
IgG 有 4 种同种型 IgG1、IgG2、IgG3 和 IgG4。它包含 4 个多肽链,由 2 条相同的 50 kDa γ 重(H)链和 2 条相同的 25 kDa κ或 λ 轻(L)链组成,它们通过链间二硫键连接。
Application
纯化的 IgG 可用作各种免疫测定中的参考抗原、标准品、封闭剂或包被蛋白,包括 ELISA、斑点免疫结合、蛋白免疫印迹、免疫扩散、免疫沉淀和免疫电泳等。其他应用包括作为制备免疫原和固相免疫吸附剂的起始材料。
Biochem/physiol Actions
IgG 介导经胎盘转运并参与二次免疫反应。IgG 激活补体系统的经典途径。它中和病毒颗粒和毒素。IgG 在抗体依赖性细胞介导的细胞毒性(ADCC)和吞噬作用中起关键作用。它可能与过敏有关。它具有最长的血清半衰期。
IgG抗体亚型是免疫系统中最丰富的血清免疫球蛋白。它由B细胞分泌,存在于血液和细胞外液中,可防止细菌、真菌和病毒引起的感染。母体IgG可通过胎盘转移至胎儿,胎盘对新生儿免疫防御感染至关重要。
Analysis Note
蛋白质浓度应在重组后通过 280 nm 处的吸光度(E1%/280 = 14)测定。
Disclaimer
除非我们的产品目录或产品附带的其他公司文档另有说明,否则我们的产品仅供研究使用,不得用于任何其他目的,包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。
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存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
低风险生物材料
此项目有
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全球贸易项目编号
| 货号 | GTIN |
|---|---|
| I5131-50MG | 04061833262580 |
| I5131-10MG | 04061833262573 |
| I5131-100MG | 04061833857120 |