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Merck
CN

I2771

ITS+3 液体培养基补充剂 (100×)

liquid, sterile-filtered, BioReagent, suitable for cell culture

别名:

ITS补充剂

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关于此项目

NACRES:
NA.75
UNSPSC Code:
12352205

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产品名称

ITS+3 液体培养基补充剂 (100×), liquid, sterile-filtered, BioReagent, suitable for cell culture

sterility

sterile-filtered

product line

BioReagent

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

shipped in

ambient

storage temp.

2-8°C

Quality Level

300

相关类别

Preparation Note

使用不含酚红的Earle′平衡盐溶液（EBSS）制备。

Application

ITS+3液体培养基补充剂(100×)作为培养基补充剂已用于：
  • 支持人脂肪干细胞(ASC)生长
  • 培养猪椎间盘(IVD)细胞和组织
  • 培养软骨终板(CEP)细胞

Biochem/physiol Actions

  • 胰岛素：一种多肽激素，可促进葡萄糖和氨基酸的摄取，可能会影响有丝分裂。
  • 转铁蛋白：铁运输蛋白。铁是一种必需微量元素，但游离形式可能有毒。为了滋养培养细胞，可与血清转铁蛋白结合后使用。
  • 硒：血清中的一种必需微量元素。

General description

ITS+3液体培养基补充剂是胰岛素、人转铁蛋白（部分铁饱和）和亚硒酸钠、亚油酸和牛血清白蛋白(BSA)的混合物。适合无血清细胞培养应用。

Other Notes

含 1.0 mg/mL 牛胰岛素、0.55 mg/mL 人转铁蛋白（基本上无铁）、0.5μg/ml 亚硒酸钠、470 μg/m亚油酸、470 μg/ml 油酸和50 mg/ml牛血清白蛋白（100x 浓度）。每摩尔白蛋白含有 2 摩尔亚油酸和油酸。

存储类别

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

高风险级别生物产品--人源产品
低风险生物材料
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000275403
0000275403
SLCL8719
SLCG4092
SLBZ3215

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