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安全信息

I2771

Sigma-Aldrich

ITS+3 液体培养基补充剂 (100×)

liquid, sterile-filtered, BioReagent, suitable for cell culture

别名:

ITS Supplement

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About This Item

UNSPSC代码:
12352205
NACRES:
NA.75

无菌性

sterile-filtered

质量水平

300

产品线

BioReagent

表单

liquid

技术

cell culture | mammalian: suitable

杂质

endotoxin, tested

运输

ambient

储存温度

2-8°C

相关类别

一般描述

ITS+3液体培养基补充剂是胰岛素、人转铁蛋白（部分铁饱和）和亚硒酸钠、亚油酸和牛血清白蛋白(BSA)的混合物。适合无血清细胞培养应用。

应用

ITS+3液体培养基补充剂(100×)作为培养基补充剂已用于：
  • 支持人脂肪干细胞(ASC)生长
  • 培养猪椎间盘(IVD)细胞和组织
  • 培养软骨终板(CEP)细胞

生化/生理作用

  • 胰岛素：一种多肽激素，可促进葡萄糖和氨基酸的摄取，可能会影响有丝分裂。
  • 转铁蛋白：铁运输蛋白。铁是一种必需微量元素，但游离形式可能有毒。为了滋养培养细胞，可与血清转铁蛋白结合后使用。
  • 硒：血清中的一种必需微量元素。

组分

含 1.0 mg/mL 牛胰岛素、0.55 mg/mL 人转铁蛋白（基本上无铁）、0.5μg/ml 亚硒酸钠、470 μg/m亚油酸、470 μg/ml 油酸和50 mg/ml牛血清白蛋白（100x 浓度）。每摩尔白蛋白含有 2 摩尔亚油酸和油酸。

制备说明

使用不含酚红的Earle′平衡盐溶液（EBSS）制备。

储存分类代码

12 - Non Combustible Liquids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

法规信息

高风险级别生物产品--人源产品

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000275403
0000275403
SLCL8719
SLCG4092
SLBZ3215

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棕榈酸等脂肪酸和其他细胞培养成分如何影响无血清、无蛋白细胞培养系统（用于生物制造包括单克隆抗体在内的异源蛋白）的性能。

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