Anti-phospho-Insulin Receptor Substrate-1 (pTyr⁶¹²) antibody produced in rabbit

Insulin receptor substrate-1 (IRS-1) is among the four IRS proteins (IRS-1-IRS-4) that mediate signalling by insulin receptor (IR) and insulin-like growth factor (IGF) receptor. IRS-1 has a pleckstrin homology domain and a phosphotyrosine-binding domain. . Phosphorylation of Tyr⁶¹² and Tyr⁶³² fully activate the PI3K signalling pathway in response to insulin and induce translocation of GLUT4. Thus IRS-1 protein plays a vital role in mediating the metabolic actions of insulin via the PI3K pathway. Additionally, IRS-1 has the abililty to translocate to the nucleus, the mechanism of which is not very clear. The transcriptional activity of IRS-1 is involved in the development of breast and prostate cancers
The antibody recognizes human IRS-1 (pTyr⁶¹²). It may cross react with in mouse, rat and chicken (100% homologous) IRS-1 (pTyr⁶¹²).

synthetic phosphopeptide derived from the region of insulin receptor substrate-1 (IRS-1) phosphorylated at tyrosine 612.

Anti-phospho-IRS-1 (pTyr⁶¹²) antibody may be used for detection by immunoblotting.

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Western Blotting (1 paper)

Solution in Dulbecco′s PBS, pH 7.3, 50% glycerol containing 1 mg/ml BSA and ≤ 0.1% sodium azide.

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