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Merck
CN

I2521

ITS + 1 液体培养基补充剂 (100×)

liquid, sterile-filtered, BioReagent, suitable for cell culture

别名:

Insulin-transferrin-sodium selenite, linoleic-BSA supplement

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NACRES:
NA.75
UNSPSC Code:
12352205

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产品名称

ITS + 1 液体培养基补充剂 (100×), liquid, sterile-filtered, BioReagent, suitable for cell culture

sterility

sterile-filtered

product line

BioReagent

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

shipped in

ambient

storage temp.

2-8°C

Quality Level

500

相关类别

Application

ITS+1液体培养基补充剂(100×)已用作高糖杜氏改良Eagle培养基(DMEM)的补充剂，用于小鼠间充质干细胞(mMSC)向软骨细胞的分化。还用作基础分化培养基的补充剂，用于在无血清条件下使人多能诱导干细胞(hiPSC)分化为成骨细胞。
胰岛素 - 转铁蛋白 - 亚硒酸钠，亚油酸 - BSA已用于无血清细胞培养应用.ITS + 1液体培养基补充剂（100×）已用于培养细胞系和用于制备永生化培养基，以用于生产来自′永生鼠′胚胎的细胞系。

General description

无血清培养可避免血清带来的成分差异和污染。无血清配方将通常由血清提供的因子替换为纯化形式，适合许多体外生长和分化研究。这些纯化因子包括胰岛素、转铁蛋白、硒、丙酮酸和乙醇胺。其他补充成分并不一定，具体取决于研究的细胞类型和所用的基础培养基。ITS补充剂是胰岛素、人转铁蛋白（部分铁饱和）和亚硒酸钠的混合物。它是一种通用的细胞补充剂，设计用于含有丙酮酸钠的非复合型培养基（如MEM、RPMI-1640）及复合型培养基（如Ham′s F-12、DME/F-12、MEM）。

Other Notes

含 1.0 mg/mL 牛胰岛素、0.55 mg/mL 人转铁蛋白（基本上无铁）、0.5μg/ml 亚硒酸钠、50 mg/mL 牛血清白蛋白和 470μg/ml 亚油酸（100x 浓度）。

Preparation Note

使用不含酚红的Earle′平衡盐溶液（EBSS）制备。

存储类别

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type ABEK (EN14387) respirator filter

法规信息

常规特殊物品
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number
0000381927
0000381927
0000322305
0000322305
SLCP1573

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