产品名称
IMAC-Select Affinity Gel, (1:1 suspension in a 30% ethanol solution)
form
(1:1 suspension in a 30% ethanol solution)
shelf life
1 yr
technique(s)
affinity chromatography: suitable
impurities
≤0.08 Metal Contamination (μmol/ml packed gel)
matrix
6% beaded agarose
capacity
10-30 μmol/mL binding capacity (Cu+2)
shipped in
wet ice
storage temp.
2-8°C
Quality Level
Application
一种固定化金属亲和层析 (IMAC) 产品，可广泛用于纯化目的蛋白、多肽和核苷酸，最常见的是蛋白。IMAC-Select 利用非电荷连接化学将专有的四齿螯合物连接到琼脂糖珠基质上。IMAC-Select Affinity Gel 是耐用的，能够以对各种金属离子（如镍、钴、铁或镓）的亲和力捕获目标蛋白。一种无金属树脂允许研究人员添加金属的选择，或使用树脂去除金属污染的蛋白质。IMAC-Select Affinity Gel 已用于 kelch 样 ECH 相关蛋白 1 (KEAP1)、X 染色体上的肾母细胞瘤基因 (WTX) 和安第斯病毒的糖蛋白 G1 尾蛋白的亲和纯化。
Other Notes
除非含有抗菌剂（如 30% 乙醇），否则不要让亲和凝胶长时间保留在任何缓冲液中。
Physical form
30% 乙醇溶液的混悬液 (1:1)
Preparation Note
在使用前，必须除去用于储存定性凝胶的乙醇，以避免缓冲盐沉淀。先用 1-2 倍体积的去离子水洗涤亲和凝胶，除去乙醇。向凝胶中加入 2-3 倍体积浓度为 10 mg/mL 的所需金属离子溶液，然后用 3-5 倍体积平衡缓冲液平衡。
signalword
Warning
hcodes
Hazard Classifications
Flam. Liq. 3
存储类别
3 - Flammable liquids
wgk
WGK 3
flash_point_f
120.2 °F - closed cup
flash_point_c
49 °C - closed cup
ppe
Eyeshields, Faceshields, Gloves, type ABEK (EN14387) respirator filter
法规信息
危险化学品
此项目有
Hao Li et al.
Proteomics, 9(6), 1646-1661 (2009-03-03)
Ethylene regulates a variety of stress responses and developmental adaptation in plants. In the present study, the phosphoproteomics is adopted to investigate the differential protein phosphorylation by ethylene in Arabidopsis ethylene-insensitive 2 (ein2) mutant. A total of 224 phosphopeptides were
Wilms tumor gene on the X chromosome (WTX) inhibits the degradation of NRF2 through competitive binding to KEAP1
Camp ND, et al.
The Journal of Biological Chemistry, jbc-M111 (2012)
The hantavirus glycoprotein G1 tail contains dual CCHC-type classical zinc fingers
Estrada DF, et al.
The Journal of Biological Chemistry, 284(13), 8654-8660 (2009)
Yishai Levin et al.
Journal of proteomics, 73(3), 689-695 (2009-11-10)
In order to exploit human blood as a source of protein disease biomarkers, robust analytical methods are needed to overcome the inherent molecular complexity of this bio-fluid. We present the coupling of label-free SAX chromatography and IMAC to a data-independent
Wilms tumor gene on the X chromosome (WTX) inhibits the degradation of NRF2 through competitive binding to KEAP1
Camp ND, et al.
Test, jbc-M111 (2012)
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