推荐产品
生物来源
mouse
质量水平
偶联物
unconjugated
抗体形式
purified immunoglobulin
抗体产品类型
primary antibodies
克隆
65.1, monoclonal
表单
buffered aqueous solution
分子量
antigen ~59 kDa
种属反应性
rat, human, bovine, monkey, mouse, canine
浓度
~2 mg/mL
技术
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 0.5-2.0 μg/mL using whole cell extract of Chinese hamster ovary cell line (CHO cells)
同位素/亚型
IgG2b
UniProt登记号
运输
dry ice
储存温度
−20°C
靶向翻译后修饰
unmodified
基因信息
human ... ILK(3611)
mouse ... Ilk(16202)
rat ... Ilk(170922)
一般描述
Monoclonal Anti-ILK (mouse IgG2b isotype) is derived from the 65.1 hybridoma produced by the fusion of mouse myeloma cells (P3X63-Ag8.653) and splenocytes from BALB/c mice immunized with purified mouse ILK recombinant protein. Integrin-linked kinase (ILK) is a ubiquitously expressed 50-59 kDa serine/threonine kinase that has three structurally well-conserved domains. A C-terminal domain contains the kinase catalytic site as well as the binding site for integrin β1 cytoplasmic domain. A N-terminal domain contains four ankyrin repeats (ANK).
免疫原
purified mouse ILK recombinant protein.
应用
Monoclonal Anti-ILK antibody produced in mouse has been used in:
- immunostaining
- immunoprecipitation
- immunocytochemistry
- immunohistochemistry
- western blotting
生化/生理作用
Integrin-linked kinase (ILK) is a serine-threonine kinase that interacts with PINCH and parvin to modulate cell adhesion, growth, differentiation, migration and invasion. This kinase binds to integrin β1, β2 and β3 domains to regulate integrin signalling. ILK functions as a receptor-proximal effector for integrin and growth factor dependent signal transduction. ILK is associated with cell cycle progression and oncogenic transformation. The kinase activity of ILK is low in non-activated cells; its activity is stimulated by cell-extracellular matrix (ECM) interactions and by certain growth factors. Negative regulation of ILK is mediated by two phosphatases: phosphatase and tensin homolog (PTEN), a tumor suppressor lipid phosphatase and ILKAP (ILK associated serine/threonine phosphatase), a protein phosphatase 2C (PP2C) protein phosphatase. In tumor cells that do not express PTEN protein, ILK is constitutively active.
外形
Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.
免责声明
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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法规信息
新产品
从最新的版本中选择一种:
Integrin-linked kinase (ILK) and its interactors: a new paradigm for the coupling of extracellular matrix to actin cytoskeleton and signaling complexes
The Journal of Cell Biology, 155(4), 505-510 (2001)
Promoter characterization and genomic organization of the gene encoding integrin-linked kinase 1
Biochimica et Biophysica Acta (BBA)-Gene Structure and Expression, 1575(1-3), 117-122 (2002)
Requirement for integrin-linked kinase in neural crest migration and differentiation and outflow tract morphogenesis
BMC Biology, 11(1), 107-107 (2013)
Kindlin-2 controls TGF-beta signalling and Sox9 expression to regulate chondrogenesis
Nature Communications, 6(1), 7531-7531 (2015)
Pinch1 is required for normal development of cranial and cardiac neural crest-derived structures
Circulation Research, 100(4), 527-535 (2007)
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