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Merck
CN

I0783

Sigma-Aldrich

Monoclonal Anti-ILK antibody produced in mouse

~2 mg/mL, clone 65.1, purified immunoglobulin, buffered aqueous solution

别名:

Anti-Integrin-linked protein kinase

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About This Item

MDL编号:
UNSPSC代码:
12352203
NACRES:
NA.44

生物来源

mouse

质量水平

偶联物

unconjugated

抗体形式

purified immunoglobulin

抗体产品类型

primary antibodies

克隆

65.1, monoclonal

表单

buffered aqueous solution

分子量

antigen ~59 kDa

种属反应性

rat, human, bovine, monkey, mouse, canine

浓度

~2 mg/mL

技术

immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
microarray: suitable
western blot: 0.5-2.0 μg/mL using whole cell extract of Chinese hamster ovary cell line (CHO cells)

同位素/亚型

IgG2b

UniProt登记号

运输

dry ice

储存温度

−20°C

靶向翻译后修饰

unmodified

基因信息

human ... ILK(3611)
mouse ... Ilk(16202)
rat ... Ilk(170922)

一般描述

Monoclonal Anti-ILK (mouse IgG2b isotype) is derived from the 65.1 hybridoma produced by the fusion of mouse myeloma cells (P3X63-Ag8.653) and splenocytes from BALB/c mice immunized with purified mouse ILK recombinant protein. Integrin-linked kinase (ILK) is a ubiquitously expressed 50-59 kDa serine/threonine kinase that has three structurally well-conserved domains. A C-terminal domain contains the kinase catalytic site as well as the binding site for integrin β1 cytoplasmic domain. A N-terminal domain contains four ankyrin repeats (ANK).

免疫原

purified mouse ILK recombinant protein.

应用

Monoclonal Anti-ILK antibody produced in mouse has been used in:
  • immunostaining
  • immunoprecipitation
  • immunocytochemistry
  • immunohistochemistry
  • western blotting

生化/生理作用

Integrin-linked kinase (ILK) is a serine-threonine kinase that interacts with PINCH and parvin to modulate cell adhesion, growth, differentiation, migration and invasion. This kinase binds to integrin β1, β2 and β3 domains to regulate integrin signalling. ILK functions as a receptor-proximal effector for integrin and growth factor dependent signal transduction. ILK is associated with cell cycle progression and oncogenic transformation. The kinase activity of ILK is low in non-activated cells; its activity is stimulated by cell-extracellular matrix (ECM) interactions and by certain growth factors. Negative regulation of ILK is mediated by two phosphatases: phosphatase and tensin homolog (PTEN), a tumor suppressor lipid phosphatase and ILKAP (ILK associated serine/threonine phosphatase), a protein phosphatase 2C (PP2C) protein phosphatase. In tumor cells that do not express PTEN protein, ILK is constitutively active.

外形

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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访问文档库

Integrin-linked kinase (ILK) and its interactors: a new paradigm for the coupling of extracellular matrix to actin cytoskeleton and signaling complexes
Wu C and Dedhar S
The Journal of Cell Biology, 155(4), 505-510 (2001)
Promoter characterization and genomic organization of the gene encoding integrin-linked kinase 1
Melchior C, et al.
Biochimica et Biophysica Acta (BBA)-Gene Structure and Expression, 1575(1-3), 117-122 (2002)
Requirement for integrin-linked kinase in neural crest migration and differentiation and outflow tract morphogenesis
Dai X, et al.
BMC Biology, 11(1), 107-107 (2013)
Kindlin-2 controls TGF-beta signalling and Sox9 expression to regulate chondrogenesis
Wu C, et al.
Nature Communications, 6(1), 7531-7531 (2015)
Pinch1 is required for normal development of cranial and cardiac neural crest-derived structures
Liang X, et al.
Circulation Research, 100(4), 527-535 (2007)

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